Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28563 | 3' | -47 | NC_005946.1 | + | 102298 | 0.66 | 0.998973 |
Target: 5'- ---cGUGCAGGGccccGCacGCCAgGGCGUCa -3' miRNA: 3'- uguuCAUGUUUU----UGa-CGGUgUCGCAG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 34820 | 0.66 | 0.998906 |
Target: 5'- cACAGGacguacuuguagccUGCAAu--CUGCUcCGGCGUCa -3' miRNA: 3'- -UGUUC--------------AUGUUuuuGACGGuGUCGCAG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 94305 | 0.66 | 0.998115 |
Target: 5'- gGCAGGUACAGgccGGACgGCguuCACGGCcuGUCu -3' miRNA: 3'- -UGUUCAUGUU---UUUGaCG---GUGUCG--CAG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 45885 | 0.67 | 0.995381 |
Target: 5'- gACAGGUACAGcgcGACgGCCACAcCGg- -3' miRNA: 3'- -UGUUCAUGUUu--UUGaCGGUGUcGCag -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 20355 | 0.67 | 0.995381 |
Target: 5'- aGCAGGcUGgAGAAGgUGuCCGCGGCGgUCg -3' miRNA: 3'- -UGUUC-AUgUUUUUgAC-GGUGUCGC-AG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 20852 | 0.69 | 0.986884 |
Target: 5'- -uGAGgGCAAAGGCUGCgGCAGaCGcCg -3' miRNA: 3'- ugUUCaUGUUUUUGACGgUGUC-GCaG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 61217 | 0.69 | 0.98504 |
Target: 5'- gACAGGUACuuuGAGCagUGCCugGGCa-- -3' miRNA: 3'- -UGUUCAUGuu-UUUG--ACGGugUCGcag -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 45530 | 0.7 | 0.980767 |
Target: 5'- cCGAGUAUugag---GCCACGGUGUCg -3' miRNA: 3'- uGUUCAUGuuuuugaCGGUGUCGCAG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 19624 | 0.7 | 0.980055 |
Target: 5'- gACAuGUcugaGCAGAccGGCUGCCccgaccugucugccGCAGCGUCu -3' miRNA: 3'- -UGUuCA----UGUUU--UUGACGG--------------UGUCGCAG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 21266 | 0.72 | 0.943157 |
Target: 5'- uACAGGUGCAuggccaccgucuuuGAGACgGCagacucuCACGGCGUCa -3' miRNA: 3'- -UGUUCAUGU--------------UUUUGaCG-------GUGUCGCAG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 71332 | 0.73 | 0.903636 |
Target: 5'- gGCuGGUAUA--GACUGCUgggACAGCGUCc -3' miRNA: 3'- -UGuUCAUGUuuUUGACGG---UGUCGCAG- -5' |
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28563 | 3' | -47 | NC_005946.1 | + | 7329 | 0.74 | 0.889169 |
Target: 5'- uGCGAGUGCAccgcGACUGCCGUGGUGUa -3' miRNA: 3'- -UGUUCAUGUuu--UUGACGGUGUCGCAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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