Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28563 | 5' | -53.8 | NC_005946.1 | + | 7904 | 0.66 | 0.927422 |
Target: 5'- -aGACGUg--ACAgcaCCUCUUGAGCCa -3' miRNA: 3'- gaCUGCGacaUGUg--GGAGAGCUUGGa -5' |
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28563 | 5' | -53.8 | NC_005946.1 | + | 67697 | 0.66 | 0.903342 |
Target: 5'- -gGACGUcGgGCcCCCUCUCG-ACCUg -3' miRNA: 3'- gaCUGCGaCaUGuGGGAGAGCuUGGA- -5' |
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28563 | 5' | -53.8 | NC_005946.1 | + | 75752 | 0.67 | 0.875239 |
Target: 5'- gUGACGCUGUcuaucaGgGCCCUCgcaauaUCGGcCCUg -3' miRNA: 3'- gACUGCGACA------UgUGGGAG------AGCUuGGA- -5' |
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28563 | 5' | -53.8 | NC_005946.1 | + | 9109 | 0.68 | 0.85736 |
Target: 5'- aUGACGCUcuuugguucaugagGaGCAUUgUCUCGGACCUg -3' miRNA: 3'- gACUGCGA--------------CaUGUGGgAGAGCUUGGA- -5' |
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28563 | 5' | -53.8 | NC_005946.1 | + | 11551 | 0.68 | 0.849219 |
Target: 5'- -aGAUGUUGUGCGCCgUUCUCGucuuaaucuucuaaAACCUc -3' miRNA: 3'- gaCUGCGACAUGUGG-GAGAGC--------------UUGGA- -5' |
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28563 | 5' | -53.8 | NC_005946.1 | + | 99735 | 0.69 | 0.780032 |
Target: 5'- cCUGACGUcccACGCCCUCUCcauGGCCa -3' miRNA: 3'- -GACUGCGacaUGUGGGAGAGc--UUGGa -5' |
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28563 | 5' | -53.8 | NC_005946.1 | + | 39541 | 0.71 | 0.709685 |
Target: 5'- uCUGACGUccacaaACACCUUCUCGGGCUUu -3' miRNA: 3'- -GACUGCGaca---UGUGGGAGAGCUUGGA- -5' |
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28563 | 5' | -53.8 | NC_005946.1 | + | 27926 | 0.71 | 0.657093 |
Target: 5'- cCUGcACGCUcucgccugGCAUCCUCUUGGGCCUc -3' miRNA: 3'- -GAC-UGCGAca------UGUGGGAGAGCUUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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