Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28565 | 5' | -60.3 | NC_005946.1 | + | 14621 | 0.66 | 0.705454 |
Target: 5'- aCCCUGC-UGGACGCuuuCCCCGGaGAGa -3' miRNA: 3'- gGGGAUGcGUCUGCG---GGGGCUgCUCc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 67435 | 0.66 | 0.705454 |
Target: 5'- aCCCCUggugGCGUagaGGugGUCCCUGACc--- -3' miRNA: 3'- -GGGGA----UGCG---UCugCGGGGGCUGcucc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 44326 | 0.66 | 0.684846 |
Target: 5'- aCCCUUGCGCuugagggcuggguGGAgGCCCCUGuuuGCGGu- -3' miRNA: 3'- -GGGGAUGCG-------------UCUgCGGGGGC---UGCUcc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 89871 | 0.66 | 0.666041 |
Target: 5'- aCCUCUGCcCGGugG-CCUCGcCGGGGa -3' miRNA: 3'- -GGGGAUGcGUCugCgGGGGCuGCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 86713 | 0.66 | 0.666041 |
Target: 5'- cUCCCUGaGCGGAgGCCaCCagGAUGAGa -3' miRNA: 3'- -GGGGAUgCGUCUgCGG-GGg-CUGCUCc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 73709 | 0.66 | 0.666041 |
Target: 5'- gCCCUACaucaaGCAGuACGUgCCCGugGGa- -3' miRNA: 3'- gGGGAUG-----CGUC-UGCGgGGGCugCUcc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 73450 | 0.66 | 0.656102 |
Target: 5'- gCCaaaUACGCAGAUcggGCCUCUGugGAagaGGa -3' miRNA: 3'- gGGg--AUGCGUCUG---CGGGGGCugCU---CC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 27778 | 0.67 | 0.646145 |
Target: 5'- cUCCCUAaaguCGUAGAUGCCgUUGGCGAa- -3' miRNA: 3'- -GGGGAU----GCGUCUGCGGgGGCUGCUcc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 41578 | 0.67 | 0.646145 |
Target: 5'- aCCCCUcCGCcgccAGACGUCCCCGcCu--- -3' miRNA: 3'- -GGGGAuGCG----UCUGCGGGGGCuGcucc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 72895 | 0.67 | 0.636177 |
Target: 5'- gUCCCUACG-AGACGCCCgaGG-GAGa -3' miRNA: 3'- -GGGGAUGCgUCUGCGGGggCUgCUCc -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 102227 | 0.68 | 0.586438 |
Target: 5'- aCCCUcACGCAGcacagcuCGgCCUCGuCGGGGg -3' miRNA: 3'- gGGGA-UGCGUCu------GCgGGGGCuGCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 96573 | 0.68 | 0.586438 |
Target: 5'- aCUCUGgugcCGcCAGACGCCa-UGACGAGGg -3' miRNA: 3'- gGGGAU----GC-GUCUGCGGggGCUGCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 86072 | 0.68 | 0.576558 |
Target: 5'- -gCUUGCGguG-CGCuCCCCuGCGAGGu -3' miRNA: 3'- ggGGAUGCguCuGCG-GGGGcUGCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 71900 | 0.68 | 0.547167 |
Target: 5'- aCCCgucgUGCGCGGGgGCCCuuGccAgGAGGg -3' miRNA: 3'- gGGG----AUGCGUCUgCGGGggC--UgCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 95333 | 0.68 | 0.537472 |
Target: 5'- cCCCCgagGCGauuucccccGGCGCCCgUgGACGAGGc -3' miRNA: 3'- -GGGGa--UGCgu-------CUGCGGG-GgCUGCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 25892 | 0.68 | 0.537472 |
Target: 5'- aCUCUAgGCc--CGUCCUCGACGGGGa -3' miRNA: 3'- gGGGAUgCGucuGCGGGGGCUGCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 73527 | 0.7 | 0.44456 |
Target: 5'- aCCCCgugGCGGAucuggccaagcaCGUCCCCGACGgcAGGu -3' miRNA: 3'- -GGGGaugCGUCU------------GCGGGGGCUGC--UCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 74998 | 0.7 | 0.435758 |
Target: 5'- gCUCCaGCGC---UGCCCCUGAUGAGGc -3' miRNA: 3'- -GGGGaUGCGucuGCGGGGGCUGCUCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 80367 | 0.72 | 0.34606 |
Target: 5'- gUCCCUGCcuucggaCAGGgGCCCuCCGACGuGGa -3' miRNA: 3'- -GGGGAUGc------GUCUgCGGG-GGCUGCuCC- -5' |
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28565 | 5' | -60.3 | NC_005946.1 | + | 79943 | 0.74 | 0.25815 |
Target: 5'- gCCCaucuggACGCcgAGGCGCCCaCGGCGGGGg -3' miRNA: 3'- -GGGga----UGCG--UCUGCGGGgGCUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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