Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28566 | 3' | -52.8 | NC_005946.1 | + | 97230 | 0.66 | 0.956123 |
Target: 5'- --gGGCUG-GGGCUCUGGgGUCUUGGg -3' miRNA: 3'- guaCUGGUaUCUGAGACUgCGGGACC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 47623 | 0.66 | 0.938177 |
Target: 5'- --aGGCuCAgGGACUCUGcCaCCCUGGc -3' miRNA: 3'- guaCUG-GUaUCUGAGACuGcGGGACC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 13116 | 0.67 | 0.927662 |
Target: 5'- --aGGuCCAUAGACUCUGAcauguaCGCCUg-- -3' miRNA: 3'- guaCU-GGUAUCUGAGACU------GCGGGacc -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 97885 | 0.67 | 0.927662 |
Target: 5'- -uUGGCCGggaGGACcCUGGCuccGUCCUGGc -3' miRNA: 3'- guACUGGUa--UCUGaGACUG---CGGGACC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 98205 | 0.67 | 0.922014 |
Target: 5'- -uUGACCAUguGGACUggGGCGgCCUGc -3' miRNA: 3'- guACUGGUA--UCUGAgaCUGCgGGACc -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 69308 | 0.67 | 0.916106 |
Target: 5'- uGUGucACCGUAGACgagCUGAcCGCCgUGc -3' miRNA: 3'- gUAC--UGGUAUCUGa--GACU-GCGGgACc -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 27431 | 0.67 | 0.916106 |
Target: 5'- --cGGCCGagaugAGGCUcCUGAggucggUGCCCUGGu -3' miRNA: 3'- guaCUGGUa----UCUGA-GACU------GCGGGACC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 67509 | 0.67 | 0.903514 |
Target: 5'- -uUGACCA-GGACgUCUGAgGCCUUucGGg -3' miRNA: 3'- guACUGGUaUCUG-AGACUgCGGGA--CC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 25529 | 0.69 | 0.859762 |
Target: 5'- uCAUGACCA-AGcuuaACUUUGACGCCUcaGGa -3' miRNA: 3'- -GUACUGGUaUC----UGAGACUGCGGGa-CC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 12399 | 0.69 | 0.859762 |
Target: 5'- --gGACCGUGuGGCUCc-ACGCCCgGGg -3' miRNA: 3'- guaCUGGUAU-CUGAGacUGCGGGaCC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 17089 | 0.69 | 0.834785 |
Target: 5'- gAUGACU--GGGCUacgaUGAgaaCGCCCUGGa -3' miRNA: 3'- gUACUGGuaUCUGAg---ACU---GCGGGACC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 20900 | 0.7 | 0.798716 |
Target: 5'- --aGGCCcugucGGcCUCaGACGCCCUGGa -3' miRNA: 3'- guaCUGGua---UCuGAGaCUGCGGGACC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 53435 | 0.7 | 0.779659 |
Target: 5'- --gGACCGUGGAC-CgugGACGCCaaagGGg -3' miRNA: 3'- guaCUGGUAUCUGaGa--CUGCGGga--CC- -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 56083 | 0.71 | 0.7399 |
Target: 5'- cCAUGGCCcucAGGCUCUccagggugaggGugGCCCUGu -3' miRNA: 3'- -GUACUGGua-UCUGAGA-----------CugCGGGACc -5' |
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28566 | 3' | -52.8 | NC_005946.1 | + | 88610 | 0.74 | 0.605954 |
Target: 5'- gCAUGGCCGUGGACgaugaguacuccauaGACGgCCUGGg -3' miRNA: 3'- -GUACUGGUAUCUGaga------------CUGCgGGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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