Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28566 | 5' | -59.3 | NC_005946.1 | + | 8250 | 0.66 | 0.662644 |
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Target: 5'- aUCCUGCAUGGCGccgucUGGccacugccugGCGUugGGUCUGc -3' miRNA: 3'- cGGGACGUGCCGC-----ACC----------UGCA--CCAGAU- -5' |
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| 28566 | 5' | -59.3 | NC_005946.1 | + | 72728 | 0.66 | 0.642099 |
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Target: 5'- gGCgCUGCGgGGUccgcguccGUGGACGUGGa--- -3' miRNA: 3'- -CGgGACGUgCCG--------CACCUGCACCagau -5' |
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| 28566 | 5' | -59.3 | NC_005946.1 | + | 72693 | 0.69 | 0.481481 |
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Target: 5'- gGUCCUGUGCGGCGUG-ACG-GGUg-- -3' miRNA: 3'- -CGGGACGUGCCGCACcUGCaCCAgau -5' |
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| 28566 | 5' | -59.3 | NC_005946.1 | + | 2096 | 0.7 | 0.44415 |
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Target: 5'- uGCCCUGCGCGGCu--GACGUcGGagagCUAa -3' miRNA: 3'- -CGGGACGUGCCGcacCUGCA-CCa---GAU- -5' |
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| 28566 | 5' | -59.3 | NC_005946.1 | + | 66936 | 0.7 | 0.417246 |
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Target: 5'- -aCCUGCAUGGCcUGGuucaagauCGUGGUCa- -3' miRNA: 3'- cgGGACGUGCCGcACCu-------GCACCAGau -5' |
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| 28566 | 5' | -59.3 | NC_005946.1 | + | 56464 | 0.72 | 0.342831 |
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Target: 5'- cGUCCUGCGgGGCGUGGACcucugGaGUCg- -3' miRNA: 3'- -CGGGACGUgCCGCACCUGca---C-CAGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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