Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28568 | 3' | -56 | NC_005946.1 | + | 35113 | 0.66 | 0.843532 |
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Target: 5'- aGGACGU-CCAUCCaccugucuaGCUGUCCC-UCCc -3' miRNA: 3'- -CUUGUAuGGUAGG---------CGACGGGGcAGGu -5' |
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| 28568 | 3' | -56 | NC_005946.1 | + | 19616 | 0.66 | 0.843532 |
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Target: 5'- uGAGCAgACCG---GCUGCCCCGaCCu -3' miRNA: 3'- -CUUGUaUGGUaggCGACGGGGCaGGu -5' |
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| 28568 | 3' | -56 | NC_005946.1 | + | 88162 | 0.66 | 0.843532 |
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Target: 5'- uGGGgGUACCGUg-GCUcagGCCCUGUCCAg -3' miRNA: 3'- -CUUgUAUGGUAggCGA---CGGGGCAGGU- -5' |
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| 28568 | 3' | -56 | NC_005946.1 | + | 49905 | 0.66 | 0.817688 |
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Target: 5'- uGGCAgggGCCAcggcaCCGCaagGCCCCGUUCc -3' miRNA: 3'- cUUGUa--UGGUa----GGCGa--CGGGGCAGGu -5' |
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| 28568 | 3' | -56 | NC_005946.1 | + | 38249 | 0.69 | 0.648466 |
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Target: 5'- cGACAgGCCAUCCGgaGCCCg--CCAa -3' miRNA: 3'- cUUGUaUGGUAGGCgaCGGGgcaGGU- -5' |
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| 28568 | 3' | -56 | NC_005946.1 | + | 103758 | 0.7 | 0.606305 |
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Target: 5'- uGACGUAcCCAUUggagggguaGCUGCCCUGUCCc -3' miRNA: 3'- cUUGUAU-GGUAGg--------CGACGGGGCAGGu -5' |
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| 28568 | 3' | -56 | NC_005946.1 | + | 82215 | 0.73 | 0.426728 |
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Target: 5'- --cCGUACCcaccggGUCCGCUGCUCaCGUCCu -3' miRNA: 3'- cuuGUAUGG------UAGGCGACGGG-GCAGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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