Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28568 | 5' | -54.6 | NC_005946.1 | + | 21387 | 0.66 | 0.902111 |
Target: 5'- cGCAGUCAGGGUaaagAGGGAGGUGg--- -3' miRNA: 3'- cCGUCAGUCCUGg---UCCCUCUAUgaga -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 88679 | 0.66 | 0.895432 |
Target: 5'- uGCccGUCAaccccucGACCAGGGAGAgccUACUCg -3' miRNA: 3'- cCGu-CAGUc------CUGGUCCCUCU---AUGAGa -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 36260 | 0.66 | 0.895432 |
Target: 5'- cGGCGGUggCGGGuCUGGGGGGAgcgGC-CUu -3' miRNA: 3'- -CCGUCA--GUCCuGGUCCCUCUa--UGaGA- -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 94597 | 0.66 | 0.894751 |
Target: 5'- gGGCAagaGgauccCAGaGACCAGGGAGAUguacgacGCUUa -3' miRNA: 3'- -CCGU---Ca----GUC-CUGGUCCCUCUA-------UGAGa -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 21514 | 0.67 | 0.845397 |
Target: 5'- aGGCGGUucucaagagccugucCAGGucaGCCAGGGAccuggACUCUg -3' miRNA: 3'- -CCGUCA---------------GUCC---UGGUCCCUcua--UGAGA- -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 76577 | 0.67 | 0.824858 |
Target: 5'- cGGUccGGUCGGGcCCGuGGGAGcgGCUg- -3' miRNA: 3'- -CCG--UCAGUCCuGGU-CCCUCuaUGAga -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 21238 | 0.69 | 0.769055 |
Target: 5'- cGGCAGacucUCAcGGCguCAGGGAGAUGCUg- -3' miRNA: 3'- -CCGUC----AGUcCUG--GUCCCUCUAUGAga -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 13851 | 0.69 | 0.759239 |
Target: 5'- aGGCccgacgCGGGucuuacauuuGCCGGGGAGGUGCUUg -3' miRNA: 3'- -CCGuca---GUCC----------UGGUCCCUCUAUGAGa -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 103108 | 0.69 | 0.739255 |
Target: 5'- cGGCAGgaccgcCGGGACCcaGGGGAGAgaagGgUCc -3' miRNA: 3'- -CCGUCa-----GUCCUGG--UCCCUCUa---UgAGa -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 49771 | 0.7 | 0.698167 |
Target: 5'- aGGUcuuguaaaAGUUGGGcACCAGGGAGGccgaggcgUACUCUu -3' miRNA: 3'- -CCG--------UCAGUCC-UGGUCCCUCU--------AUGAGA- -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 17239 | 0.7 | 0.698167 |
Target: 5'- aGGUuGUCAGaGGCCAGGGGGcuGUAC-Ca -3' miRNA: 3'- -CCGuCAGUC-CUGGUCCCUC--UAUGaGa -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 31546 | 0.72 | 0.575799 |
Target: 5'- cGGCAGUCAGGaACCacauagaccuguugaGGGGAGcUACg-- -3' miRNA: 3'- -CCGUCAGUCC-UGG---------------UCCCUCuAUGaga -5' |
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28568 | 5' | -54.6 | NC_005946.1 | + | 71162 | 0.74 | 0.470333 |
Target: 5'- uGGCGGgCAGGuCCAGGGAGGgggGCa-- -3' miRNA: 3'- -CCGUCaGUCCuGGUCCCUCUa--UGaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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