miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28569 3' -40.4 NC_005946.1 + 101369 0.72 0.999909
Target:  5'- -aUUAgACACAAGGGUAUUgucaaccCAGACa -3'
miRNA:   3'- aaAGUgUGUGUUUCUAUAAau-----GUCUG- -5'
28569 3' -40.4 NC_005946.1 + 63477 0.71 0.999951
Target:  5'- --cCACguGCACAAAGAUGUc-ACAGGCc -3'
miRNA:   3'- aaaGUG--UGUGUUUCUAUAaaUGUCUG- -5'
28569 3' -40.4 NC_005946.1 + 59413 0.69 0.999999
Target:  5'- -aUgGCGCACGAacugaGGAUGUUUGCAaACu -3'
miRNA:   3'- aaAgUGUGUGUU-----UCUAUAAAUGUcUG- -5'
28569 3' -40.4 NC_005946.1 + 33686 0.68 1
Target:  5'- ---uGCACGCAAAGAUGU----AGACu -3'
miRNA:   3'- aaagUGUGUGUUUCUAUAaaugUCUG- -5'
28569 3' -40.4 NC_005946.1 + 15440 0.68 1
Target:  5'- -aUCgGCAUaagagugaggACGAGGAcGUUUGCAGACa -3'
miRNA:   3'- aaAG-UGUG----------UGUUUCUaUAAAUGUCUG- -5'
28569 3' -40.4 NC_005946.1 + 94320 0.66 1
Target:  5'- -aUCAgGCugAGGGAgggcaggUACAGGCc -3'
miRNA:   3'- aaAGUgUGugUUUCUauaa---AUGUCUG- -5'
28569 3' -40.4 NC_005946.1 + 7507 0.66 1
Target:  5'- aUUUUuCGCAguAGGAcuccUGUUUGCGGGCa -3'
miRNA:   3'- -AAAGuGUGUguUUCU----AUAAAUGUCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.