miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28569 5' -42.6 NC_005946.1 + 46528 0.66 1
Target:  5'- -aGUCUGUAAAUGUcgUUGcAUCUCa -3'
miRNA:   3'- ggCAGACAUUUAUAuaGAUuUGGGGa -5'
28569 5' -42.6 NC_005946.1 + 56388 0.69 0.999965
Target:  5'- gCCGUCUGUGAcgGccgaGUCcagGAGCCCg- -3'
miRNA:   3'- -GGCAGACAUUuaUa---UAGa--UUUGGGga -5'
28569 5' -42.6 NC_005946.1 + 26783 0.71 0.999665
Target:  5'- cCUGUCUG-AGGUAgGUCU--ACCCCa -3'
miRNA:   3'- -GGCAGACaUUUAUaUAGAuuUGGGGa -5'
28569 5' -42.6 NC_005946.1 + 42209 0.72 0.999144
Target:  5'- gCCGUCcacuAcgAUGUCUGGACCCUg -3'
miRNA:   3'- -GGCAGacauUuaUAUAGAUUUGGGGa -5'
28569 5' -42.6 NC_005946.1 + 81285 0.73 0.997881
Target:  5'- uCUGUCUGUAAAguuucagcacaGUCUcAGCCCCa -3'
miRNA:   3'- -GGCAGACAUUUaua--------UAGAuUUGGGGa -5'
28569 5' -42.6 NC_005946.1 + 80604 0.78 0.952222
Target:  5'- aCCGUCUGagAGAUuaaGUCUGAGCCUCa -3'
miRNA:   3'- -GGCAGACa-UUUAua-UAGAUUUGGGGa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.