Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28570 | 5' | -55.8 | NC_005946.1 | + | 49519 | 0.66 | 0.854509 |
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Target: 5'- gGGUgCACG-AUCCCUCcCUGauGGAGAUu -3' miRNA: 3'- -CCAaGUGCgUAGGGAG-GAC--CCUCUGc -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 75779 | 0.67 | 0.838248 |
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Target: 5'- aGGgagCACGCGacgucUCCCUUCUuGGuGACGc -3' miRNA: 3'- -CCaa-GUGCGU-----AGGGAGGAcCCuCUGC- -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 40539 | 0.67 | 0.794354 |
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Target: 5'- -uUUUAUGCAUCUUUUUUGGGAGAa- -3' miRNA: 3'- ccAAGUGCGUAGGGAGGACCCUCUgc -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 34924 | 0.68 | 0.775677 |
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Target: 5'- ---aCAUGUccaGUCCCUCCUgaccGGGAGgACGg -3' miRNA: 3'- ccaaGUGCG---UAGGGAGGA----CCCUC-UGC- -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 73628 | 0.68 | 0.73681 |
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Target: 5'- uGggCugGgGUCUUUUUUGGGAGACGa -3' miRNA: 3'- cCaaGugCgUAGGGAGGACCCUCUGC- -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 14916 | 0.69 | 0.726834 |
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Target: 5'- cGG-UCACGUAggCCgUCUuguggcccgcuaUGGGAGACGg -3' miRNA: 3'- -CCaAGUGCGUa-GGgAGG------------ACCCUCUGC- -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 56320 | 0.69 | 0.716773 |
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Target: 5'- uGGUcCuCGCGUCCaUCCuggagacaaggUGGGAGACGu -3' miRNA: 3'- -CCAaGuGCGUAGGgAGG-----------ACCCUCUGC- -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 74796 | 0.71 | 0.613605 |
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Target: 5'- gGGUUCAC-CAggcCCCUCUU-GGAGGCGu -3' miRNA: 3'- -CCAAGUGcGUa--GGGAGGAcCCUCUGC- -5' |
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| 28570 | 5' | -55.8 | NC_005946.1 | + | 19398 | 0.75 | 0.391252 |
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Target: 5'- cGUUCACGg--CCCugugccugccuaUCCUGGGAGACGc -3' miRNA: 3'- cCAAGUGCguaGGG------------AGGACCCUCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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