miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28572 5' -51.7 NC_005946.1 + 8090 0.75 0.554085
Target:  5'- gGGUGgcCaGGAuGAGCUCGGGAGGCAUc -3'
miRNA:   3'- -CCGCaaGaCCU-CUCGAGUUUUCCGUA- -5'
28572 5' -51.7 NC_005946.1 + 56455 0.67 0.918384
Target:  5'- gGGCGUggaccUCUGGAGucgaugggguGCUCAu-GGGCu- -3'
miRNA:   3'- -CCGCA-----AGACCUCu---------CGAGUuuUCCGua -5'
28572 5' -51.7 NC_005946.1 + 75640 0.67 0.940567
Target:  5'- --gGUUCUGGAGGGuCUCGAGuuuGGaCAg -3'
miRNA:   3'- ccgCAAGACCUCUC-GAGUUUu--CC-GUa -5'
28572 5' -51.7 NC_005946.1 + 81484 0.66 0.94543
Target:  5'- aGGCagaggaUCUGGAGAGCaUCA--AGGCc- -3'
miRNA:   3'- -CCGca----AGACCUCUCG-AGUuuUCCGua -5'
28572 5' -51.7 NC_005946.1 + 86874 0.67 0.918384
Target:  5'- aGGacugUCUGGGGAGCagUCAGGGGaGCGUc -3'
miRNA:   3'- -CCgca-AGACCUCUCG--AGUUUUC-CGUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.