Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28581 | 5' | -56.6 | NC_005965.1 | + | 1848 | 0.66 | 0.109838 |
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Target: 5'- gGGuGACGuaGCUUCCAAacacgccGcCGCCGACCCa -3' miRNA: 3'- -CC-UUGCc-CGGAGGUU-------CaGUGGCUGGG- -5' |
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| 28581 | 5' | -56.6 | NC_005965.1 | + | 1292 | 0.66 | 0.106609 |
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Target: 5'- aGAACGGGaCCUCCGcucccgGGUCAagugaagGugCCg -3' miRNA: 3'- cCUUGCCC-GGAGGU------UCAGUgg-----CugGG- -5' |
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| 28581 | 5' | -56.6 | NC_005965.1 | + | 2821 | 0.68 | 0.076277 |
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Target: 5'- cGAACGcGGaucuguacUCUCCuuacuaucucAAGUCAUCGACCCu -3' miRNA: 3'- cCUUGC-CC--------GGAGG----------UUCAGUGGCUGGG- -5' |
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| 28581 | 5' | -56.6 | NC_005965.1 | + | 3315 | 0.7 | 0.058148 |
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Target: 5'- cGAuauCGGGCCUCCucugaGAGaUCGgCgGGCCCa -3' miRNA: 3'- cCUu--GCCCGGAGG-----UUC-AGU-GgCUGGG- -5' |
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| 28581 | 5' | -56.6 | NC_005965.1 | + | 700 | 0.75 | 0.02067 |
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Target: 5'- uGGACaGGCCaUCCGGGUUACCGcACUCg -3' miRNA: 3'- cCUUGcCCGG-AGGUUCAGUGGC-UGGG- -5' |
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| 28581 | 5' | -56.6 | NC_005965.1 | + | 1361 | 1.13 | 0.000012 |
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Target: 5'- uGGAACGGGCCUCCAAGUCACCGACCCc -3' miRNA: 3'- -CCUUGCCCGGAGGUUCAGUGGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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