Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2871 | 3' | -55.2 | NC_001493.1 | + | 119434 | 0.66 | 0.914884 |
Target: 5'- -aUGAGC-CGGuCCGUCCG-GGGUg-- -3' miRNA: 3'- caACUCGuGCU-GGUAGGCgCCCAagc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 3880 | 0.66 | 0.914884 |
Target: 5'- -aUGAGC-CGGuCCGUCCG-GGGUg-- -3' miRNA: 3'- caACUCGuGCU-GGUAGGCgCCCAagc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 67810 | 0.67 | 0.882467 |
Target: 5'- gGUUGAGaaucaACuuguGACUGUUCGuCGGGUUCGa -3' miRNA: 3'- -CAACUCg----UG----CUGGUAGGC-GCCCAAGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 54704 | 0.67 | 0.882467 |
Target: 5'- --cGAuGCGCGcGCCAUCCGUccGGUUCa -3' miRNA: 3'- caaCU-CGUGC-UGGUAGGCGc-CCAAGc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 34264 | 0.67 | 0.875289 |
Target: 5'- --cGAGCACGuggguggguccGCCAgCUGCcGGUUCGg -3' miRNA: 3'- caaCUCGUGC-----------UGGUaGGCGcCCAAGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 99846 | 0.67 | 0.860274 |
Target: 5'- -gUGGGC-CcACC-UCCGUGGGUUCc -3' miRNA: 3'- caACUCGuGcUGGuAGGCGCCCAAGc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 62585 | 0.68 | 0.844421 |
Target: 5'- --cGGGCACGGCCAcggCCaCGGGcaCGg -3' miRNA: 3'- caaCUCGUGCUGGUa--GGcGCCCaaGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 110200 | 0.68 | 0.827782 |
Target: 5'- gGUUGAGC--GACCAUgaGCGGGaaggUCGa -3' miRNA: 3'- -CAACUCGugCUGGUAggCGCCCa---AGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 119348 | 0.68 | 0.825222 |
Target: 5'- --gGAGCGCGcCCcugugcgaaccgguAUUCGCGGGggCGg -3' miRNA: 3'- caaCUCGUGCuGG--------------UAGGCGCCCaaGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 3794 | 0.68 | 0.825222 |
Target: 5'- --gGAGCGCGcCCcugugcgaaccgguAUUCGCGGGggCGg -3' miRNA: 3'- caaCUCGUGCuGG--------------UAGGCGCCCaaGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 9082 | 0.68 | 0.822646 |
Target: 5'- -aUGAGCGCGACCcgggucuuuugcgcgGUcaacgggagCCGCGGGgUCa -3' miRNA: 3'- caACUCGUGCUGG---------------UA---------GGCGCCCaAGc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 124637 | 0.68 | 0.822646 |
Target: 5'- -aUGAGCGCGACCcgggucuuuugcgcgGUcaacgggagCCGCGGGgUCa -3' miRNA: 3'- caACUCGUGCUGG---------------UA---------GGCGCCCaAGc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 131643 | 0.69 | 0.810419 |
Target: 5'- --cGAGUACGggGCCGUCC-CGGGgcUCGa -3' miRNA: 3'- caaCUCGUGC--UGGUAGGcGCCCa-AGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 16088 | 0.69 | 0.810419 |
Target: 5'- --cGAGUACGggGCCGUCC-CGGGgcUCGa -3' miRNA: 3'- caaCUCGUGC--UGGUAGGcGCCCa-AGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 69252 | 0.69 | 0.810419 |
Target: 5'- gGUUGAaCAgGGCCcuGUCCGUcacgGGGUUCGa -3' miRNA: 3'- -CAACUcGUgCUGG--UAGGCG----CCCAAGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 130554 | 0.69 | 0.773787 |
Target: 5'- --gGGGUGCGAUCGaggCCGCGGGUg-- -3' miRNA: 3'- caaCUCGUGCUGGUa--GGCGCCCAagc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 14999 | 0.69 | 0.773787 |
Target: 5'- --gGGGUGCGAUCGaggCCGCGGGUg-- -3' miRNA: 3'- caaCUCGUGCUGGUa--GGCGCCCAagc -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 27124 | 0.72 | 0.633291 |
Target: 5'- --cGGGUGCGuGCCAUUC-CGGGUUCGg -3' miRNA: 3'- caaCUCGUGC-UGGUAGGcGCCCAAGC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 133657 | 0.74 | 0.51141 |
Target: 5'- cUUGAGCACGGCCAUucCCGUGGcGgacagCGg -3' miRNA: 3'- cAACUCGUGCUGGUA--GGCGCC-Caa---GC- -5' |
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2871 | 3' | -55.2 | NC_001493.1 | + | 18102 | 0.74 | 0.51141 |
Target: 5'- cUUGAGCACGGCCAUucCCGUGGcGgacagCGg -3' miRNA: 3'- cAACUCGUGCUGGUA--GGCGCC-Caa---GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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