Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28727 | 3' | -53.1 | NC_006146.1 | + | 167316 | 0.69 | 0.898218 |
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Target: 5'- uGCAGggggggcCGGCGGgGCgucccGUCGUCAcGCUCGg -3' miRNA: 3'- -CGUC-------GCCGCUgUGa----UAGUAGU-CGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 170111 | 0.7 | 0.877512 |
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Target: 5'- cGCAGggggggcCGGCGGgGCgucccGUCGUCAcGCUCGg -3' miRNA: 3'- -CGUC-------GCCGCUgUGa----UAGUAGU-CGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 128282 | 0.7 | 0.855578 |
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Target: 5'- aCGGCGGCGGCcgagccccagGCUucgGUCAGCUCu -3' miRNA: 3'- cGUCGCCGCUG----------UGAuagUAGUCGAGc -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 152896 | 0.7 | 0.855578 |
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Target: 5'- -gGGUGGCGAUGCcGUC--CAGCUCGa -3' miRNA: 3'- cgUCGCCGCUGUGaUAGuaGUCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 107909 | 0.72 | 0.777236 |
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Target: 5'- -aAGUGGCGAaaaCAUgggagGUCGUCAGCUCc -3' miRNA: 3'- cgUCGCCGCU---GUGa----UAGUAGUCGAGc -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 130297 | 0.75 | 0.636587 |
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Target: 5'- aGCAGCGGCGGCAUg--CGgaGGUUCGg -3' miRNA: 3'- -CGUCGCCGCUGUGauaGUagUCGAGC- -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 81586 | 0.77 | 0.524069 |
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Target: 5'- -aAGCGGcCGACACUAcauUCAgagCAGCUCa -3' miRNA: 3'- cgUCGCC-GCUGUGAU---AGUa--GUCGAGc -5' |
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| 28727 | 3' | -53.1 | NC_006146.1 | + | 168390 | 1.11 | 0.004395 |
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Target: 5'- cGCAGCGGCGACACUAUCAUCAGCUCGu -3' miRNA: 3'- -CGUCGCCGCUGUGAUAGUAGUCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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