Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2873 | 3' | -63.8 | NC_001493.1 | + | 60083 | 0.66 | 0.506176 |
Target: 5'- gGUGACCGugacGGGUUGGCGaGGUcGAGg- -3' miRNA: 3'- -CACUGGCcc--CCCGACCGC-CCA-CUCag -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 9263 | 0.66 | 0.496886 |
Target: 5'- -gGACCGGGGaGGCgGGCGuGGggcugGAcuaccugcGUCu -3' miRNA: 3'- caCUGGCCCC-CCGaCCGC-CCa----CU--------CAG- -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 124817 | 0.66 | 0.496886 |
Target: 5'- -gGACCGGGGaGGCgGGCGuGGggcugGAcuaccugcGUCu -3' miRNA: 3'- caCUGGCCCC-CCGaCCGC-CCa----CU--------CAG- -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 14109 | 0.66 | 0.487675 |
Target: 5'- cGUGAUCGuGGGGGUggUGGUGGcGaUGGGg- -3' miRNA: 3'- -CACUGGC-CCCCCG--ACCGCC-C-ACUCag -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 129664 | 0.66 | 0.487675 |
Target: 5'- cGUGAUCGuGGGGGUggUGGUGGcGaUGGGg- -3' miRNA: 3'- -CACUGGC-CCCCCG--ACCGCC-C-ACUCag -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 19603 | 0.66 | 0.487675 |
Target: 5'- -aGugCGGGGGGUgacgugggGGCcuauaucagGGGUGGGa- -3' miRNA: 3'- caCugGCCCCCCGa-------CCG---------CCCACUCag -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 24099 | 0.67 | 0.451676 |
Target: 5'- ----aCGGGGGGCUGGCGa-UGuGUCc -3' miRNA: 3'- cacugGCCCCCCGACCGCccACuCAG- -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 36623 | 0.68 | 0.41718 |
Target: 5'- gGUGGCCGuGGGGCU-GUGGGUGuccagGGUa -3' miRNA: 3'- -CACUGGCcCCCCGAcCGCCCAC-----UCAg -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 111828 | 0.68 | 0.376402 |
Target: 5'- -cGACCGGGGcugguGCUcGCGGGUacgcccgaaGAGUCg -3' miRNA: 3'- caCUGGCCCCc----CGAcCGCCCA---------CUCAG- -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 102475 | 0.7 | 0.310058 |
Target: 5'- gGUGGaaGGuGGGGCcucGGCGGGUcgggGGGUCg -3' miRNA: 3'- -CACUggCC-CCCCGa--CCGCCCA----CUCAG- -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 115709 | 0.71 | 0.276596 |
Target: 5'- -aGACCccaaGGGGGCgugggggUGGCgaGGGUGGGUCu -3' miRNA: 3'- caCUGGc---CCCCCG-------ACCG--CCCACUCAG- -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 120850 | 1.07 | 0.000652 |
Target: 5'- gGUGACCGGGGGGCUGGCGGGUGAGUCc -3' miRNA: 3'- -CACUGGCCCCCCGACCGCCCACUCAG- -5' |
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2873 | 3' | -63.8 | NC_001493.1 | + | 5296 | 1.07 | 0.000652 |
Target: 5'- gGUGACCGGGGGGCUGGCGGGUGAGUCc -3' miRNA: 3'- -CACUGGCCCCCCGACCGCCCACUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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