Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28732 | 5' | -52 | NC_006146.1 | + | 129533 | 0.67 | 0.971134 |
Target: 5'- gCCGGaUGCCcca-CCAgAGGACACGUGu -3' miRNA: 3'- -GGUC-ACGGuagaGGUgUUCUGUGCAU- -5' |
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28732 | 5' | -52 | NC_006146.1 | + | 131389 | 0.66 | 0.985098 |
Target: 5'- cCCGGUGCUuUCUCCcCGuguccuccucAGACAUGa- -3' miRNA: 3'- -GGUCACGGuAGAGGuGU----------UCUGUGCau -5' |
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28732 | 5' | -52 | NC_006146.1 | + | 142298 | 0.7 | 0.894265 |
Target: 5'- uUCAGUaUguUCUCCACAGGGCAgGUGu -3' miRNA: 3'- -GGUCAcGguAGAGGUGUUCUGUgCAU- -5' |
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28732 | 5' | -52 | NC_006146.1 | + | 166412 | 1.09 | 0.007072 |
Target: 5'- gCCAGUGCCAUCUCCACAAGACACGUAa -3' miRNA: 3'- -GGUCACGGUAGAGGUGUUCUGUGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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