Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28735 | 5' | -46.6 | NC_006146.1 | + | 163091 | 0.68 | 0.99894 |
Target: 5'- aUCCUAAuuuAGUUGGGuaguguauacuAuccuagcuuucggUAGCAUAUGCUa -3' miRNA: 3'- cAGGAUU---UCAACCC-----------U-------------AUCGUAUACGA- -5' |
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28735 | 5' | -46.6 | NC_006146.1 | + | 163197 | 0.66 | 0.999835 |
Target: 5'- uUCCUAAuuuAGUUGGGuaguguauacuAuccuagcuuucggUAGCAUAUGCUn -3' miRNA: 3'- cAGGAUU---UCAACCC-----------U-------------AUCGUAUACGA- -5' |
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28735 | 5' | -46.6 | NC_006146.1 | + | 163250 | 0.87 | 0.40468 |
Target: 5'- aUCCUAAuuuAGUUGGG-UAGCAUAUGCUa -3' miRNA: 3'- cAGGAUU---UCAACCCuAUCGUAUACGA- -5' |
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28735 | 5' | -46.6 | NC_006146.1 | + | 163278 | 0.87 | 0.40468 |
Target: 5'- aUCCUAAuuuAGUUGGG-UAGCAUAUGCUa -3' miRNA: 3'- cAGGAUU---UCAACCCuAUCGUAUACGA- -5' |
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28735 | 5' | -46.6 | NC_006146.1 | + | 163334 | 0.74 | 0.960899 |
Target: 5'- aUCCUAGcuUUGGG-UAGCAUAUGUUn -3' miRNA: 3'- cAGGAUUucAACCCuAUCGUAUACGA- -5' |
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28735 | 5' | -46.6 | NC_006146.1 | + | 163359 | 0.77 | 0.857036 |
Target: 5'- aUCCUGAuauGUUaGGGUAGUAUAUGCUa -3' miRNA: 3'- cAGGAUUu--CAAcCCUAUCGUAUACGA- -5' |
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28735 | 5' | -46.6 | NC_006146.1 | + | 163387 | 0.66 | 0.999906 |
Target: 5'- aUCCUAGuGUaaccuuuuGAUAGCAUAUGCUa -3' miRNA: 3'- cAGGAUUuCAacc-----CUAUCGUAUACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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