miRNA: 3'- gCuCUGCG-CGG----AGUGCUCUUCUCCGC- -5'
miRNA: 3'- gCUCUGCGCGGAGUgCUCUU-CU--CCGC- -5'
miRNA: 3'- gCUCUGCGCGGAGUgCUCUU-CU--CCGC- -5'
miRNA: 3'- -GCUCUGCGCGGAGUGCUCUUCUCCGC- -5'
miRNA: 3'- -GCUCUGCGCGGAGUGCUCUUCUCCGC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2874 | 5' | -56.9 | NC_001493.1 | + | 108779 | 0.73 | 0.494935 |
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Target: 5'- aGcGGCGCgGCUccgaUCACGGGAGGAGGUGc -3' miRNA: 3'- gCuCUGCG-CGG----AGUGCUCUUCUCCGC- -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 120766 | 0.73 | 0.48544 |
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Target: 5'- uGAGGCGCGUCUCG-GGGAAcGAauGGCGg -3' miRNA: 3'- gCUCUGCGCGGAGUgCUCUU-CU--CCGC- -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 5212 | 0.73 | 0.48544 |
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Target: 5'- uGAGGCGCGUCUCG-GGGAAcGAauGGCGg -3' miRNA: 3'- gCUCUGCGCGGAGUgCUCUU-CU--CCGC- -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 120914 | 1.08 | 0.002634 |
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Target: 5'- cCGAGACGCGCCUCACGAGAAGAGGCGc -3' miRNA: 3'- -GCUCUGCGCGGAGUGCUCUUCUCCGC- -5' |
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| 2874 | 5' | -56.9 | NC_001493.1 | + | 5360 | 1.08 | 0.002634 |
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Target: 5'- cCGAGACGCGCCUCACGAGAAGAGGCGc -3' miRNA: 3'- -GCUCUGCGCGGAGUGCUCUUCUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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