Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28740 | 3' | -54.8 | NC_006146.1 | + | 119119 | 0.66 | 0.932011 |
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Target: 5'- aGUgGCU-GGACUCgGCUcugGGCAGAGAg -3' miRNA: 3'- cUAgCGAgUCUGAGaCGG---UCGUCUCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 37914 | 0.66 | 0.932011 |
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Target: 5'- --gCGCUgGGGgaCUGCUaAGCAGGGAc -3' miRNA: 3'- cuaGCGAgUCUgaGACGG-UCGUCUCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 128206 | 0.66 | 0.932011 |
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Target: 5'- cGggCGCUgggGGGCUCUGCCAGCcucAGGc -3' miRNA: 3'- -CuaGCGAg--UCUGAGACGGUCGuc-UCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 123596 | 0.66 | 0.937045 |
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Target: 5'- ---aGCUCAaagaaGCUCUuggccaGCCAGCGGAGGu -3' miRNA: 3'- cuagCGAGUc----UGAGA------CGGUCGUCUCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 62131 | 0.66 | 0.937045 |
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Target: 5'- uAUgGCccUCAGAauUUCUGCCAGCcucAGGGAa -3' miRNA: 3'- cUAgCG--AGUCU--GAGACGGUCG---UCUCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 110875 | 0.66 | 0.937045 |
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Target: 5'- --gCGuCUCGGGCUCggGCgcaGGCGGGGAc -3' miRNA: 3'- cuaGC-GAGUCUGAGa-CGg--UCGUCUCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 40497 | 0.66 | 0.941835 |
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Target: 5'- --cCGCgaaaggcagCGGACUCUGCCu-CGGAGGc -3' miRNA: 3'- cuaGCGa--------GUCUGAGACGGucGUCUCU- -5' |
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| 28740 | 3' | -54.8 | NC_006146.1 | + | 125217 | 0.66 | 0.946382 |
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Target: 5'- cGggCGCUCAGAgcccUUCcGCUcccuGGCGGAGAc -3' miRNA: 3'- -CuaGCGAGUCU----GAGaCGG----UCGUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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