Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28746 | 3' | -54.8 | NC_006146.1 | + | 70611 | 0.75 | 0.50042 |
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Target: 5'- uGAUCGCggugCAGGC-CUGCCAGCccccgcuaGGAGGc -3' miRNA: 3'- -CUAGCGa---GUCUGaGACGGUCG--------UCUCU- -5' |
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| 28746 | 3' | -54.8 | NC_006146.1 | + | 117596 | 0.72 | 0.632663 |
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Target: 5'- ---gGCUCGGACUCcgGCCAG-GGAGAg -3' miRNA: 3'- cuagCGAGUCUGAGa-CGGUCgUCUCU- -5' |
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| 28746 | 3' | -54.8 | NC_006146.1 | + | 126184 | 0.71 | 0.724997 |
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Target: 5'- --cCGCaUCAGGC-CgGCCAGCGGGGGc -3' miRNA: 3'- cuaGCG-AGUCUGaGaCGGUCGUCUCU- -5' |
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| 28746 | 3' | -54.8 | NC_006146.1 | + | 48117 | 0.68 | 0.87576 |
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Target: 5'- -uUCGCg-AGACUCUGUCGcCGGGGAg -3' miRNA: 3'- cuAGCGagUCUGAGACGGUcGUCUCU- -5' |
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| 28746 | 3' | -54.8 | NC_006146.1 | + | 128206 | 0.66 | 0.932011 |
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Target: 5'- cGggCGCUgggGGGCUCUGCCAGCcucAGGc -3' miRNA: 3'- -CuaGCGAg--UCUGAGACGGUCGuc-UCU- -5' |
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| 28746 | 3' | -54.8 | NC_006146.1 | + | 62131 | 0.66 | 0.937045 |
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Target: 5'- uAUgGCccUCAGAauUUCUGCCAGCcucAGGGAa -3' miRNA: 3'- cUAgCG--AGUCU--GAGACGGUCG---UCUCU- -5' |
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| 28746 | 3' | -54.8 | NC_006146.1 | + | 110875 | 0.66 | 0.937045 |
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Target: 5'- --gCGuCUCGGGCUCggGCgcaGGCGGGGAc -3' miRNA: 3'- cuaGC-GAGUCUGAGa-CGg--UCGUCUCU- -5' |
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| 28746 | 3' | -54.8 | NC_006146.1 | + | 40497 | 0.66 | 0.941835 |
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Target: 5'- --cCGCgaaaggcagCGGACUCUGCCu-CGGAGGc -3' miRNA: 3'- cuaGCGa--------GUCUGAGACGGucGUCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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