Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2875 | 3' | -55.4 | NC_001493.1 | + | 128600 | 0.66 | 0.895111 |
Target: 5'- --gGGGUCAacagCUGGUgGAGcGAGUGCGu -3' miRNA: 3'- cgaCCCAGUg---GAUCAaCUC-CUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 13046 | 0.66 | 0.895111 |
Target: 5'- --gGGGUCAacagCUGGUgGAGcGAGUGCGu -3' miRNA: 3'- cgaCCCAGUg---GAUCAaCUC-CUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 50962 | 0.66 | 0.881234 |
Target: 5'- -gUGGGUCGC--GGUUGguGGGGGUGCc -3' miRNA: 3'- cgACCCAGUGgaUCAAC--UCCUCGCGu -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 119363 | 0.66 | 0.873949 |
Target: 5'- cGUUGaGUCGCCc----GAGGAGCGCGc -3' miRNA: 3'- -CGACcCAGUGGaucaaCUCCUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 3809 | 0.66 | 0.873949 |
Target: 5'- cGUUGaGUCGCCc----GAGGAGCGCGc -3' miRNA: 3'- -CGACcCAGUGGaucaaCUCCUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 124783 | 0.67 | 0.842624 |
Target: 5'- --cGGGUCGCgCUcaucGGGGAGCGCu -3' miRNA: 3'- cgaCCCAGUG-GAucaaCUCCUCGCGu -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 9229 | 0.67 | 0.842624 |
Target: 5'- --cGGGUCGCgCUcaucGGGGAGCGCu -3' miRNA: 3'- cgaCCCAGUG-GAucaaCUCCUCGCGu -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 102220 | 0.7 | 0.70144 |
Target: 5'- --aGGGUCGCgUGGcUGAuGAGCGCGc -3' miRNA: 3'- cgaCCCAGUGgAUCaACUcCUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 121572 | 0.77 | 0.307636 |
Target: 5'- aGCUGGGUCACCUAGUUGAGa------ -3' miRNA: 3'- -CGACCCAGUGGAUCAACUCcucgcgu -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 6018 | 0.77 | 0.307636 |
Target: 5'- aGCUGGGUCACCUAGUUGAGa------ -3' miRNA: 3'- -CGACCCAGUGGAUCAACUCcucgcgu -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 121373 | 0.94 | 0.027503 |
Target: 5'- aGCU-GGUCACCUAGUUGAGGAGCGUAc -3' miRNA: 3'- -CGAcCCAGUGGAUCAACUCCUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 5819 | 0.94 | 0.027503 |
Target: 5'- aGCU-GGUCACCUAGUUGAGGAGCGUAc -3' miRNA: 3'- -CGAcCCAGUGGAUCAACUCCUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 121487 | 1.1 | 0.002113 |
Target: 5'- aGCUGGGUCACCUAGUUGAGGAGCGCAc -3' miRNA: 3'- -CGACCCAGUGGAUCAACUCCUCGCGU- -5' |
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2875 | 3' | -55.4 | NC_001493.1 | + | 5933 | 1.1 | 0.002113 |
Target: 5'- aGCUGGGUCACCUAGUUGAGGAGCGCAc -3' miRNA: 3'- -CGACCCAGUGGAUCAACUCCUCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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