Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2875 | 5' | -50.3 | NC_001493.1 | + | 52285 | 0.66 | 0.994963 |
Target: 5'- gGGUUCUCUCUuuUCGGGuaucuCUAGGGA-UUCa -3' miRNA: 3'- -CCAGGAGAGA--AGCUU-----GAUCCUUgGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 46475 | 0.66 | 0.994139 |
Target: 5'- gGGUCCU-UCUUCGGACcaucgucGGGAACg-- -3' miRNA: 3'- -CCAGGAgAGAAGCUUGa------UCCUUGgag -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 73792 | 0.66 | 0.991 |
Target: 5'- -uUCCUCuUCUucUCGAAgaAGGAcuuCCUCg -3' miRNA: 3'- ccAGGAG-AGA--AGCUUgaUCCUu--GGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 69074 | 0.67 | 0.989424 |
Target: 5'- cGGUCCUCUUgUCGAACcccgugacggacAGGGcCCUg -3' miRNA: 3'- -CCAGGAGAGaAGCUUGa-----------UCCUuGGAg -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 84390 | 0.67 | 0.988259 |
Target: 5'- -cUCCUCUCUaCcauCUGGGGagACCUCa -3' miRNA: 3'- ccAGGAGAGAaGcuuGAUCCU--UGGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 51185 | 0.67 | 0.986666 |
Target: 5'- gGGcUCCgUCUCUUCGuACcGGGAgaggGCCUUc -3' miRNA: 3'- -CC-AGG-AGAGAAGCuUGaUCCU----UGGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 2954 | 0.67 | 0.986666 |
Target: 5'- -cUCCUCUCcagUGAACUcGGAAaugcCCUCg -3' miRNA: 3'- ccAGGAGAGaa-GCUUGAuCCUU----GGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 118508 | 0.67 | 0.986666 |
Target: 5'- -cUCCUCUCcagUGAACUcGGAAaugcCCUCg -3' miRNA: 3'- ccAGGAGAGaa-GCUUGAuCCUU----GGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 42716 | 0.67 | 0.986666 |
Target: 5'- gGGUCCUCUCguggUC-AGgaGGGAACUg- -3' miRNA: 3'- -CCAGGAGAGa---AGcUUgaUCCUUGGag -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 105022 | 0.67 | 0.982987 |
Target: 5'- uGUCaacUUCUUCGGGaagGGGGACCUCc -3' miRNA: 3'- cCAGga-GAGAAGCUUga-UCCUUGGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 90835 | 0.68 | 0.973402 |
Target: 5'- cGG-CCUCUCgacUCgGAGCcGGcGGGCCUCg -3' miRNA: 3'- -CCaGGAGAGa--AG-CUUGaUC-CUUGGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 130435 | 0.68 | 0.973402 |
Target: 5'- gGGUgCUCUCgUCGAGaCgcgAGGGGCCcCg -3' miRNA: 3'- -CCAgGAGAGaAGCUU-Ga--UCCUUGGaG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 14881 | 0.68 | 0.973402 |
Target: 5'- gGGUgCUCUCgUCGAGaCgcgAGGGGCCcCg -3' miRNA: 3'- -CCAgGAGAGaAGCUU-Ga--UCCUUGGaG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 24400 | 0.7 | 0.94816 |
Target: 5'- uGGUCUUUUCUUCGgGACUcAGGGucGCCa- -3' miRNA: 3'- -CCAGGAGAGAAGC-UUGA-UCCU--UGGag -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 8261 | 0.7 | 0.928278 |
Target: 5'- gGGUCCUCUCUcUCGGGggaGGGAACgUa -3' miRNA: 3'- -CCAGGAGAGA-AGCUUga-UCCUUGgAg -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 123815 | 0.7 | 0.928278 |
Target: 5'- gGGUCCUCUCUcUCGGGggaGGGAACgUa -3' miRNA: 3'- -CCAGGAGAGA-AGCUUga-UCCUUGgAg -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 119481 | 0.71 | 0.916778 |
Target: 5'- ---aCUCUU-UCGggUUGGGAACCUCc -3' miRNA: 3'- ccagGAGAGaAGCuuGAUCCUUGGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 3927 | 0.71 | 0.916778 |
Target: 5'- ---aCUCUU-UCGggUUGGGAACCUCc -3' miRNA: 3'- ccagGAGAGaAGCuuGAUCCUUGGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 6014 | 0.72 | 0.860642 |
Target: 5'- gGGUCaC-CUaguugaGAACUAGGAACCUCg -3' miRNA: 3'- -CCAG-GaGAgaag--CUUGAUCCUUGGAG- -5' |
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2875 | 5' | -50.3 | NC_001493.1 | + | 121568 | 0.72 | 0.860642 |
Target: 5'- gGGUCaC-CUaguugaGAACUAGGAACCUCg -3' miRNA: 3'- -CCAG-GaGAgaag--CUUGAUCCUUGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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