Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28756 | 3' | -56.9 | NC_006146.1 | + | 15046 | 0.66 | 0.854258 |
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Target: 5'- cAGGGCccgccggGCCUGCgagGAGGCAcuGGCggggGCu -3' miRNA: 3'- -UCCCG-------UGGAUGag-CUCCGU--CCGaa--UG- -5' |
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| 28756 | 3' | -56.9 | NC_006146.1 | + | 13205 | 0.7 | 0.638774 |
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Target: 5'- cGGGCGCCUcCUCGGguucagccgcuGGCuGGCUcUGCc -3' miRNA: 3'- uCCCGUGGAuGAGCU-----------CCGuCCGA-AUG- -5' |
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| 28756 | 3' | -56.9 | NC_006146.1 | + | 12852 | 0.7 | 0.659226 |
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Target: 5'- aGGaGGCGCCUGgggCGAGGCuggguGGCUggGCa -3' miRNA: 3'- -UC-CCGUGGAUga-GCUCCGu----CCGAa-UG- -5' |
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| 28756 | 3' | -56.9 | NC_006146.1 | + | 11848 | 0.73 | 0.507931 |
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Target: 5'- cGGGC-CCUgcuucucggGCUUGGGGCGGGCccgACg -3' miRNA: 3'- uCCCGuGGA---------UGAGCUCCGUCCGaa-UG- -5' |
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| 28756 | 3' | -56.9 | NC_006146.1 | + | 3981 | 0.67 | 0.83906 |
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Target: 5'- gAGGGCACCaaguggccaUGgUCGAGGCGcugaugaacgcGGCcuUUGCc -3' miRNA: 3'- -UCCCGUGG---------AUgAGCUCCGU-----------CCG--AAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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