Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28758 | 5' | -53 | NC_006146.1 | + | 10460 | 0.66 | 0.978798 |
Target: 5'- aGCGCCgCC--UUCGUgAUGGGg-GCCAc -3' miRNA: 3'- -CGUGG-GGuuAAGCAgUAUCCgaCGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 21328 | 0.7 | 0.848162 |
Target: 5'- cCGCCCCuuccaagaGUCAUucucggagGGGCUGCCGg -3' miRNA: 3'- cGUGGGGuuaag---CAGUA--------UCCGACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 41208 | 0.69 | 0.907338 |
Target: 5'- gGCGCCCCAuccgCGggCAcUGGGCgcgcgugGCCAg -3' miRNA: 3'- -CGUGGGGUuaa-GCa-GU-AUCCGa------CGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 46441 | 0.66 | 0.981024 |
Target: 5'- gGC-CCCCAGgaaggCGgcggCGUAGGCcggaugcgUGCCGa -3' miRNA: 3'- -CGuGGGGUUaa---GCa---GUAUCCG--------ACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 47725 | 0.66 | 0.976384 |
Target: 5'- aGCGCCCgGAgcucgUCGUCcgGGGCcuCCu -3' miRNA: 3'- -CGUGGGgUUa----AGCAGuaUCCGacGGu -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 47805 | 0.7 | 0.880392 |
Target: 5'- aGCGCCCaccgcCGUCucaugggcucGGGCUGCCAg -3' miRNA: 3'- -CGUGGGguuaaGCAGua--------UCCGACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 55451 | 0.66 | 0.976384 |
Target: 5'- -aGCUCCAGgacUCuGUCAUGGGCagcaaGCCAc -3' miRNA: 3'- cgUGGGGUUa--AG-CAGUAUCCGa----CGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 56562 | 0.66 | 0.973775 |
Target: 5'- gGC-CCCCGuccaccucCGUCAgGGGCgGCCGg -3' miRNA: 3'- -CGuGGGGUuaa-----GCAGUaUCCGaCGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 81162 | 0.66 | 0.970962 |
Target: 5'- -aGCCCCAGcgcgaaagCGUC--GGGCUGUCGu -3' miRNA: 3'- cgUGGGGUUaa------GCAGuaUCCGACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 102520 | 0.73 | 0.740385 |
Target: 5'- cCACCCCcg-UCGUCAUcaAGGCccugguUGCCAc -3' miRNA: 3'- cGUGGGGuuaAGCAGUA--UCCG------ACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 102924 | 0.83 | 0.240605 |
Target: 5'- uGCGCCCCAAga--UCGUGGGCUGCCu -3' miRNA: 3'- -CGUGGGGUUaagcAGUAUCCGACGGu -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 109108 | 0.71 | 0.824648 |
Target: 5'- gGCGCgCCCAcUUCuGUaacgAGGCUGCCGu -3' miRNA: 3'- -CGUG-GGGUuAAG-CAgua-UCCGACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 110022 | 0.69 | 0.919356 |
Target: 5'- -gGCCCCAGgcCGgucugCAgGGGCUGUCAu -3' miRNA: 3'- cgUGGGGUUaaGCa----GUaUCCGACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 112811 | 0.66 | 0.96794 |
Target: 5'- uGCGCCCCAcgUacagauaggUGUCGUAGuaGCgcaggGCCGa -3' miRNA: 3'- -CGUGGGGUuaA---------GCAGUAUC--CGa----CGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 115333 | 0.69 | 0.919356 |
Target: 5'- gGCACCCCGGacUCgGUCAUcgAGGCcuuccugGCCGg -3' miRNA: 3'- -CGUGGGGUUa-AG-CAGUA--UCCGa------CGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 122314 | 0.66 | 0.981024 |
Target: 5'- cCACCCCca-UCGUCu--GGCucuugUGCCAg -3' miRNA: 3'- cGUGGGGuuaAGCAGuauCCG-----ACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 127380 | 0.66 | 0.981024 |
Target: 5'- cGCGCCCCucGUggaggUGUCuugGGGCUggGCCc -3' miRNA: 3'- -CGUGGGGu-UAa----GCAGua-UCCGA--CGGu -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 130891 | 0.71 | 0.841599 |
Target: 5'- -uGCCCCGucugaccgGUCAaAGGCUGCCGg -3' miRNA: 3'- cgUGGGGUuaag----CAGUaUCCGACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 131477 | 0.69 | 0.894342 |
Target: 5'- uGCACCCaGGUggGUC-UGGGgUGCCAg -3' miRNA: 3'- -CGUGGGgUUAagCAGuAUCCgACGGU- -5' |
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28758 | 5' | -53 | NC_006146.1 | + | 132744 | 0.7 | 0.887484 |
Target: 5'- -aGCCCCGGUUC-UCcgGGGCcggaUGCCGc -3' miRNA: 3'- cgUGGGGUUAAGcAGuaUCCG----ACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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