Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28758 | 5' | -53 | NC_006146.1 | + | 10460 | 0.66 | 0.978798 |
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Target: 5'- aGCGCCgCC--UUCGUgAUGGGg-GCCAc -3' miRNA: 3'- -CGUGG-GGuuAAGCAgUAUCCgaCGGU- -5' |
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| 28758 | 5' | -53 | NC_006146.1 | + | 170687 | 0.66 | 0.981024 |
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Target: 5'- gGCGCCCCGGggacCGUCGcggGGGCaccgGCg- -3' miRNA: 3'- -CGUGGGGUUaa--GCAGUa--UCCGa---CGgu -5' |
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| 28758 | 5' | -53 | NC_006146.1 | + | 46441 | 0.66 | 0.981024 |
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Target: 5'- gGC-CCCCAGgaaggCGgcggCGUAGGCcggaugcgUGCCGa -3' miRNA: 3'- -CGuGGGGUUaa---GCa---GUAUCCG--------ACGGU- -5' |
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| 28758 | 5' | -53 | NC_006146.1 | + | 127380 | 0.66 | 0.981024 |
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Target: 5'- cGCGCCCCucGUggaggUGUCuugGGGCUggGCCc -3' miRNA: 3'- -CGUGGGGu-UAa----GCAGua-UCCGA--CGGu -5' |
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| 28758 | 5' | -53 | NC_006146.1 | + | 122314 | 0.66 | 0.981024 |
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Target: 5'- cCACCCCca-UCGUCu--GGCucuugUGCCAg -3' miRNA: 3'- cGUGGGGuuaAGCAGuauCCG-----ACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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