Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28765 | 5' | -48.4 | NC_006146.1 | + | 98456 | 0.66 | 0.999357 |
Target: 5'- cGCC---GCCcGGgggCCAGUCUcuggGUCAGu -3' miRNA: 3'- -CGGauuUGGaCCa--GGUCAGAa---UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 131240 | 0.67 | 0.999025 |
Target: 5'- cGCCcccaGAGCCUGGaucUCCAGcCUcAUCAc -3' miRNA: 3'- -CGGa---UUUGGACC---AGGUCaGAaUAGUc -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 101051 | 0.67 | 0.999005 |
Target: 5'- uGCCUGcGCCUGcGUgacccgcuucucgCCGGUCUgggugGUCAa -3' miRNA: 3'- -CGGAUuUGGAC-CA-------------GGUCAGAa----UAGUc -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 25779 | 0.67 | 0.998555 |
Target: 5'- gGCCaGAGCCUGGcCCAGgccuUCUUcAUCu- -3' miRNA: 3'- -CGGaUUUGGACCaGGUC----AGAA-UAGuc -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 125368 | 0.68 | 0.9975 |
Target: 5'- uGCCUGGcCCUGGUCaCGuUCUUGUgCAu -3' miRNA: 3'- -CGGAUUuGGACCAG-GUcAGAAUA-GUc -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 132668 | 0.69 | 0.994694 |
Target: 5'- cGCCgAGACg-GGUCCAGUUUUAcgcugcgugaauucuUCAGg -3' miRNA: 3'- -CGGaUUUGgaCCAGGUCAGAAU---------------AGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 59254 | 0.69 | 0.993455 |
Target: 5'- gGCCUGgguguucagGGCCUGG-CCGacGUCUUcgCGGa -3' miRNA: 3'- -CGGAU---------UUGGACCaGGU--CAGAAuaGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 27641 | 0.69 | 0.993162 |
Target: 5'- cGCUUcuugcucuucacagGAACCUggGGUCUAGUCUgggugggAUCAGg -3' miRNA: 3'- -CGGA--------------UUUGGA--CCAGGUCAGAa------UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 24563 | 0.69 | 0.993162 |
Target: 5'- cGCUUcuugcucuucacagGAACCUggGGUCUAGUCUgggugggAUCAGg -3' miRNA: 3'- -CGGA--------------UUUGGA--CCAGGUCAGAa------UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 21485 | 0.69 | 0.993162 |
Target: 5'- cGCUUcuugcucuucacagGAACCUggGGUCUAGUCUgggugggAUCAGg -3' miRNA: 3'- -CGGA--------------UUUGGA--CCAGGUCAGAa------UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 18407 | 0.69 | 0.993162 |
Target: 5'- cGCUUcuugcucuucacagGAACCUggGGUCUAGUCUgggugggAUCAGg -3' miRNA: 3'- -CGGA--------------UUUGGA--CCAGGUCAGAa------UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 15329 | 0.69 | 0.993162 |
Target: 5'- cGCUUcuugcucuucacagGAACCUggGGUCUAGUCUgggugggAUCAGg -3' miRNA: 3'- -CGGA--------------UUUGGA--CCAGGUCAGAa------UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 12250 | 0.69 | 0.993162 |
Target: 5'- cGCUUcuugcucuucacagGAACCUggGGUCUAGUCUgggugggAUCAGg -3' miRNA: 3'- -CGGA--------------UUUGGA--CCAGGUCAGAa------UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 157256 | 0.7 | 0.990023 |
Target: 5'- gGCCgcuGCCUGuGUCCGGcgCUUgaucGUCAGc -3' miRNA: 3'- -CGGauuUGGAC-CAGGUCa-GAA----UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 85480 | 0.7 | 0.990023 |
Target: 5'- gGCCUucacGGCCUGG-CUGGUCUccucGUCGGg -3' miRNA: 3'- -CGGAu---UUGGACCaGGUCAGAa---UAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 60804 | 0.7 | 0.990023 |
Target: 5'- uCCaGGGCCUGGUCCGGaCcgUUGUCAa -3' miRNA: 3'- cGGaUUUGGACCAGGUCaG--AAUAGUc -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 116595 | 0.71 | 0.976625 |
Target: 5'- gGCCUGAGCCUGGccacacgucgcuUCCuGGUC---UCAGg -3' miRNA: 3'- -CGGAUUUGGACC------------AGG-UCAGaauAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 60418 | 0.72 | 0.967974 |
Target: 5'- uCCUGGACCUGG-CCGGgguggaaUGUCAGa -3' miRNA: 3'- cGGAUUUGGACCaGGUCaga----AUAGUC- -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 110117 | 0.73 | 0.948899 |
Target: 5'- cCCUGGACCUGGUCCGG-CUc----- -3' miRNA: 3'- cGGAUUUGGACCAGGUCaGAauaguc -5' |
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28765 | 5' | -48.4 | NC_006146.1 | + | 132386 | 1.13 | 0.010584 |
Target: 5'- gGCCUAAACCUGGUCCAGUCUUAUCAGa -3' miRNA: 3'- -CGGAUUUGGACCAGGUCAGAAUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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