Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28766 | 3' | -56 | NC_006146.1 | + | 166006 | 0.66 | 0.90635 |
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Target: 5'- -cGGCUCugucaccaGGCGgCGCAGGC-CGAGa -3' miRNA: 3'- uaCCGAGug------UCGCaGUGUUCGcGCUC- -5' |
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| 28766 | 3' | -56 | NC_006146.1 | + | 167782 | 0.68 | 0.836258 |
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Target: 5'- uUGGCggggagaaugacagCugGGCGUgGCGGGCGCGc- -3' miRNA: 3'- uACCGa-------------GugUCGCAgUGUUCGCGCuc -5' |
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| 28766 | 3' | -56 | NC_006146.1 | + | 168714 | 0.68 | 0.836258 |
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Target: 5'- uUGGCggggagaaugacagCugGGCGUgGCGGGCGCGc- -3' miRNA: 3'- uACCGa-------------GugUCGCAgUGUUCGCGCuc -5' |
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| 28766 | 3' | -56 | NC_006146.1 | + | 169647 | 0.68 | 0.829554 |
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Target: 5'- uUGGCggggagaauacagCugGGCGUgGCGGGCGCGc- -3' miRNA: 3'- uACCGa------------GugUCGCAgUGUUCGCGCuc -5' |
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| 28766 | 3' | -56 | NC_006146.1 | + | 170578 | 0.67 | 0.852482 |
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Target: 5'- uUGGCcgggagaaugacagCugGGCGUgGCGAGCGCGccGGg -3' miRNA: 3'- uACCGa-------------GugUCGCAgUGUUCGCGC--UC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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