Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28766 | 3' | -56 | NC_006146.1 | + | 79619 | 0.71 | 0.650226 |
Target: 5'- gGUGaGCUCgucccggggACAGgGUCACAGGCcgGCGAGc -3' miRNA: 3'- -UAC-CGAG---------UGUCgCAGUGUUCG--CGCUC- -5' |
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28766 | 3' | -56 | NC_006146.1 | + | 68175 | 0.71 | 0.639921 |
Target: 5'- -gGGCaggCGCAGCGUCAgGA-CGCGGGc -3' miRNA: 3'- uaCCGa--GUGUCGCAGUgUUcGCGCUC- -5' |
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28766 | 3' | -56 | NC_006146.1 | + | 135326 | 0.71 | 0.63992 |
Target: 5'- aAUGGCUCugAGgGgaggCACGGGCGCc-- -3' miRNA: 3'- -UACCGAGugUCgCa---GUGUUCGCGcuc -5' |
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28766 | 3' | -56 | NC_006146.1 | + | 41689 | 0.75 | 0.451006 |
Target: 5'- cUGGCUCuuuCAGCGcUCACcGGCGgGGGg -3' miRNA: 3'- uACCGAGu--GUCGC-AGUGuUCGCgCUC- -5' |
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28766 | 3' | -56 | NC_006146.1 | + | 130859 | 1.06 | 0.004432 |
Target: 5'- cAUGGCUCACAGCGUCACAAGCGCGAGc -3' miRNA: 3'- -UACCGAGUGUCGCAGUGUUCGCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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