Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28770 | 3' | -55.5 | NC_006146.1 | + | 123001 | 0.68 | 0.842961 |
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Target: 5'- cGGGGGacuucuucUCGgAGCUGCUGaccgagGCcGAGGCg -3' miRNA: 3'- -CCCCC--------AGCgUCGAUGACg-----CGaCUUUG- -5' |
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| 28770 | 3' | -55.5 | NC_006146.1 | + | 88241 | 0.68 | 0.842961 |
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Target: 5'- cGGGGGUCGguGgUAC-GgGCUGu--- -3' miRNA: 3'- -CCCCCAGCguCgAUGaCgCGACuuug -5' |
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| 28770 | 3' | -55.5 | NC_006146.1 | + | 66718 | 0.69 | 0.809068 |
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Target: 5'- uGGGGG-CGCAGaCggccCUGCGaCUGGAucuGCa -3' miRNA: 3'- -CCCCCaGCGUC-Gau--GACGC-GACUU---UG- -5' |
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| 28770 | 3' | -55.5 | NC_006146.1 | + | 60278 | 0.69 | 0.807301 |
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Target: 5'- cGGGGagcgggaguuugCGgAGCUGCUGaGCUGGGACc -3' miRNA: 3'- cCCCCa-----------GCgUCGAUGACgCGACUUUG- -5' |
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| 28770 | 3' | -55.5 | NC_006146.1 | + | 60714 | 0.69 | 0.800171 |
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Target: 5'- uGGGGcCGCAuCUccaACcgGCGCUGAGGCc -3' miRNA: 3'- cCCCCaGCGUcGA---UGa-CGCGACUUUG- -5' |
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| 28770 | 3' | -55.5 | NC_006146.1 | + | 161748 | 0.69 | 0.794759 |
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Target: 5'- cGGGGGUgGCGGCUgagguccgagggggcGcCUGgGCgGggGCc -3' miRNA: 3'- -CCCCCAgCGUCGA---------------U-GACgCGaCuuUG- -5' |
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| 28770 | 3' | -55.5 | NC_006146.1 | + | 77888 | 0.7 | 0.772599 |
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Target: 5'- aGGGGUCggaGCAcGCUGCUuaacagGCGCaGggGCg -3' miRNA: 3'- cCCCCAG---CGU-CGAUGA------CGCGaCuuUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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