Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28772 | 3' | -58.1 | NC_006146.1 | + | 45335 | 0.69 | 0.671706 |
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Target: 5'- gUUGCG-GGGAUUCgG-GGGAGGCc- -3' miRNA: 3'- gGACGCaCCCUAAGgUgCCCUCCGau -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 40254 | 0.7 | 0.631635 |
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Target: 5'- aCCUcGgGUGGGA-UCCGuaguaGGGAGGCg- -3' miRNA: 3'- -GGA-CgCACCCUaAGGUg----CCCUCCGau -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 39745 | 0.69 | 0.70146 |
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Target: 5'- gCUG-GUGGGuUUCgGCuGGAGGCUGg -3' miRNA: 3'- gGACgCACCCuAAGgUGcCCUCCGAU- -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 39512 | 0.68 | 0.755469 |
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Target: 5'- gCCUGCuGUGGGuggcugaugggCUggGGGAGGCg- -3' miRNA: 3'- -GGACG-CACCCuaa--------GGugCCCUCCGau -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 39349 | 0.66 | 0.833144 |
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Target: 5'- uCCUGCGauucuuuggauguucUGGG--UCC-CGGGAGGgUGa -3' miRNA: 3'- -GGACGC---------------ACCCuaAGGuGCCCUCCgAU- -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 33237 | 0.66 | 0.845957 |
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Target: 5'- --gGC-UGGGGUUCCGgGGGuggGGGCUc -3' miRNA: 3'- ggaCGcACCCUAAGGUgCCC---UCCGAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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