Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28772 | 3' | -58.1 | NC_006146.1 | + | 139112 | 0.7 | 0.641671 |
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Target: 5'- gUUGCGUGGGGUgUCAauuGGGGGCUGu -3' miRNA: 3'- gGACGCACCCUAaGGUgc-CCUCCGAU- -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 47985 | 0.7 | 0.637658 |
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Target: 5'- --aGCGUGGGGUcagagguggcuggUgGCGGGAGGCUu -3' miRNA: 3'- ggaCGCACCCUAa------------GgUGCCCUCCGAu -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 117687 | 0.7 | 0.631635 |
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Target: 5'- gCUGCGUGGGAUUCUccagcauuuugAUGGcagccGAGGUUGu -3' miRNA: 3'- gGACGCACCCUAAGG-----------UGCC-----CUCCGAU- -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 40254 | 0.7 | 0.631635 |
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Target: 5'- aCCUcGgGUGGGA-UCCGuaguaGGGAGGCg- -3' miRNA: 3'- -GGA-CgCACCCUaAGGUg----CCCUCCGau -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 51205 | 0.72 | 0.532431 |
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Target: 5'- gCUGUGgagcaUGGGAgguugCUugGGGGGGCUGa -3' miRNA: 3'- gGACGC-----ACCCUaa---GGugCCCUCCGAU- -5' |
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| 28772 | 3' | -58.1 | NC_006146.1 | + | 126446 | 1.08 | 0.002466 |
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Target: 5'- uCCUGCGUGGGAUUCCACGGGAGGCUAu -3' miRNA: 3'- -GGACGCACCCUAAGGUGCCCUCCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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