Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2878 | 5' | -55.5 | NC_001493.1 | + | 25265 | 0.66 | 0.914774 |
Target: 5'- -gGCCCUGUucGGGAaCCGaggGCGACGa -3' miRNA: 3'- caUGGGGCA--CCCUaGGCacaUGCUGU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 96051 | 0.66 | 0.908787 |
Target: 5'- -cACCCCGUGacGUUCGgGUGCGugAa -3' miRNA: 3'- caUGGGGCACccUAGGCaCAUGCugU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 47930 | 0.66 | 0.902562 |
Target: 5'- cGUGCCCCGUGaaccucguGGUCCGcacgcauagGUACGAgAa -3' miRNA: 3'- -CAUGGGGCACc-------CUAGGCa--------CAUGCUgU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 54858 | 0.67 | 0.867973 |
Target: 5'- uGUGCCCCG-GGGAgcgaGUgGUGCGAg- -3' miRNA: 3'- -CAUGGGGCaCCCUagg-CA-CAUGCUgu -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 18603 | 0.67 | 0.851814 |
Target: 5'- -cACCCCGUauaacaccggaacGGGccCCGaGUACGACGg -3' miRNA: 3'- caUGGGGCA-------------CCCuaGGCaCAUGCUGU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 37443 | 0.68 | 0.828057 |
Target: 5'- uUGCCCaugGGGAUCUG-GUGCGAg- -3' miRNA: 3'- cAUGGGgcaCCCUAGGCaCAUGCUgu -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 62820 | 0.68 | 0.828057 |
Target: 5'- --cCCCCGUGGGcuucgCCGUc-GCGACGg -3' miRNA: 3'- cauGGGGCACCCua---GGCAcaUGCUGU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 49744 | 0.68 | 0.819504 |
Target: 5'- gGUGuCCCCGUGGGGUugaacaucucggCCGUGc-UGACGg -3' miRNA: 3'- -CAU-GGGGCACCCUA------------GGCACauGCUGU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 43282 | 0.69 | 0.792848 |
Target: 5'- -cGCCCCGUGG---UCGUGUACuGCAg -3' miRNA: 3'- caUGGGGCACCcuaGGCACAUGcUGU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 111912 | 0.72 | 0.603672 |
Target: 5'- -cGCCCCGUGGuGAUCacggGUGUcCGAUAc -3' miRNA: 3'- caUGGGGCACC-CUAGg---CACAuGCUGU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 124686 | 1.09 | 0.003081 |
Target: 5'- cGUACCCCGUGGGAUCCGUGUACGACAg -3' miRNA: 3'- -CAUGGGGCACCCUAGGCACAUGCUGU- -5' |
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2878 | 5' | -55.5 | NC_001493.1 | + | 9132 | 1.09 | 0.003081 |
Target: 5'- cGUACCCCGUGGGAUCCGUGUACGACAg -3' miRNA: 3'- -CAUGGGGCACCCUAGGCACAUGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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