miRNA: 3'- cuGCuGACCUUG--UGGGAAgaCCGGAg -5'
miRNA: 3'- cUGCUgACCUUGUGGGa-AGA----CCGGAG- -5'
miRNA: 3'- cuGCUGaCCUUG------UGGGAAGACC-GGAG- -5'
miRNA: 3'- -CUGCUGAccuUGUGGgAa---GACCGGAG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28781 | 3' | -55.6 | NC_006146.1 | + | 64592 | 0.69 | 0.772599 |
|
Target: 5'- -gUGuCUGGAACugGCCCUUgaGGCCUg -3' miRNA: 3'- cuGCuGACCUUG--UGGGAAgaCCGGAg -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 11914 | 0.7 | 0.734117 |
|
Target: 5'- cGCGG-UGGAGCACCUggUCUuugcGGCCUCc -3' miRNA: 3'- cUGCUgACCUUGUGGGa-AGA----CCGGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 78403 | 0.7 | 0.72425 |
|
Target: 5'- -cUGAUaGGAGCcgcuggGCCCUUCUGGaCCUCc -3' miRNA: 3'- cuGCUGaCCUUG------UGGGAAGACC-GGAG- -5' |
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| 28781 | 3' | -55.6 | NC_006146.1 | + | 115503 | 0.71 | 0.714304 |
|
Target: 5'- gGGCGACU---ACGCCgUgaugCUGGCCUCg -3' miRNA: 3'- -CUGCUGAccuUGUGGgAa---GACCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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