Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28787 | 5' | -46.7 | NC_006146.1 | + | 68185 | 0.67 | 0.999825 |
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Target: 5'- aGCgucaggacgCGGGCGCAGCGgcucCGGGAGGUc -3' miRNA: 3'- gCGa--------GCUCGCGUUGUaa--GUUUUCUAa -5' |
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| 28787 | 5' | -46.7 | NC_006146.1 | + | 58104 | 0.67 | 0.999535 |
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Target: 5'- aCGUg-GGGCGCAACGggaggCAGGAGAg- -3' miRNA: 3'- -GCGagCUCGCGUUGUaa---GUUUUCUaa -5' |
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| 28787 | 5' | -46.7 | NC_006146.1 | + | 120799 | 0.68 | 0.999417 |
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Target: 5'- uGCUCGAGCuccGCAACcggagUCAGAuccAGGUg -3' miRNA: 3'- gCGAGCUCG---CGUUGua---AGUUU---UCUAa -5' |
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| 28787 | 5' | -46.7 | NC_006146.1 | + | 122486 | 0.71 | 0.992542 |
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Target: 5'- uGCUCGGGgacgGCGGCGgggUCGAGAGAa- -3' miRNA: 3'- gCGAGCUCg---CGUUGUa--AGUUUUCUaa -5' |
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| 28787 | 5' | -46.7 | NC_006146.1 | + | 67160 | 0.77 | 0.879857 |
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Target: 5'- cCGCUCcuGAGCGCGGCGUcCGGGAGGa- -3' miRNA: 3'- -GCGAG--CUCGCGUUGUAaGUUUUCUaa -5' |
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| 28787 | 5' | -46.7 | NC_006146.1 | + | 120140 | 1.07 | 0.02808 |
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Target: 5'- cCGCUCGAGCGCAACAUUCAAAAGAUUg -3' miRNA: 3'- -GCGAGCUCGCGUUGUAAGUUUUCUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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