miRNA: 3'- uuGAGGAguCGGGGGAGUUCg-CCGau -5'
miRNA: 3'- uuGAGGAGUCGGGGGAGuu-CgCCGau -5'
Results 61 - 62 of 62 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28789 | 3' | -60 | NC_006146.1 | + | 160648 | 0.66 | 0.708222 |
|
Target: 5'- -uCUCCUguGCUUCCUCAGGgaGGCc- -3' miRNA: 3'- uuGAGGAguCGGGGGAGUUCg-CCGau -5' |
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| 28789 | 3' | -60 | NC_006146.1 | + | 163000 | 0.68 | 0.576853 |
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Target: 5'- -uCUCCUCGGCCUCCUCuuuG-GGCc- -3' miRNA: 3'- uuGAGGAGUCGGGGGAGuu-CgCCGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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