Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2879 | 3' | -57.7 | NC_001493.1 | + | 88129 | 0.66 | 0.837342 |
Target: 5'- cUCCgaGUCUCGAuCGAuCGC-UCCagGCGCc -3' miRNA: 3'- -AGGa-CAGGGCU-GCU-GCGuAGGa-CGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 81815 | 0.66 | 0.836529 |
Target: 5'- aCCgcuaCUCGACGAUGCcgCCUuuaacgaGCGCa -3' miRNA: 3'- aGGaca-GGGCUGCUGCGuaGGA-------CGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 95410 | 0.66 | 0.828299 |
Target: 5'- uUCCUGcccaCCCGugaccGCGACGCGaauauuucucgggUCCaugGCGCc -3' miRNA: 3'- -AGGACa---GGGC-----UGCUGCGU-------------AGGa--CGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 40045 | 0.66 | 0.81219 |
Target: 5'- gCUUGgaacucggaaCCCGA--GCGgGUCCUGCGCa -3' miRNA: 3'- aGGACa---------GGGCUgcUGCgUAGGACGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 45866 | 0.67 | 0.794612 |
Target: 5'- gCCUcUCCuCGGCGAgaugUGCGggCUGCGCg -3' miRNA: 3'- aGGAcAGG-GCUGCU----GCGUagGACGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 79341 | 0.67 | 0.784695 |
Target: 5'- aCCgagCgCGACGAaaacgaucaucagUGUGUCCUGCGCg -3' miRNA: 3'- aGGacaGgGCUGCU-------------GCGUAGGACGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 58881 | 0.68 | 0.719265 |
Target: 5'- gCCgGUCgCGAUGGCaCAUCCcUGCGUc -3' miRNA: 3'- aGGaCAGgGCUGCUGcGUAGG-ACGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 40236 | 0.68 | 0.699522 |
Target: 5'- uUUCUGUCCUGAgcuCGCGaaUCgUGCGCa -3' miRNA: 3'- -AGGACAGGGCUgcuGCGU--AGgACGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 79122 | 0.69 | 0.689563 |
Target: 5'- uUCCagGUCCUGgaccaGCGuguuCGCGUCC-GCGCa -3' miRNA: 3'- -AGGa-CAGGGC-----UGCu---GCGUAGGaCGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 25454 | 0.71 | 0.568744 |
Target: 5'- cUCCUGUCCCGAaaaaGACGgGcgCCUGaGUu -3' miRNA: 3'- -AGGACAGGGCUg---CUGCgUa-GGACgCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 39926 | 0.72 | 0.499381 |
Target: 5'- aCCUGUCCaGACG-CGCAUauguacacuggaaCCUGCGg -3' miRNA: 3'- aGGACAGGgCUGCuGCGUA-------------GGACGCg -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 48515 | 0.73 | 0.426979 |
Target: 5'- uUUCUGUCCgcCGGCGuCGCGUCCaccgUGCGUu -3' miRNA: 3'- -AGGACAGG--GCUGCuGCGUAGG----ACGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 92191 | 0.74 | 0.409651 |
Target: 5'- uUCCUGcUCCUGACGACGgAUCCcGgGg -3' miRNA: 3'- -AGGAC-AGGGCUGCUGCgUAGGaCgCg -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 124954 | 1.12 | 0.001146 |
Target: 5'- gUCCUGUCCCGACGACGCAUCCUGCGCa -3' miRNA: 3'- -AGGACAGGGCUGCUGCGUAGGACGCG- -5' |
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2879 | 3' | -57.7 | NC_001493.1 | + | 9400 | 1.12 | 0.001146 |
Target: 5'- gUCCUGUCCCGACGACGCAUCCUGCGCa -3' miRNA: 3'- -AGGACAGGGCUGCUGCGUAGGACGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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