Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28793 | 3' | -55.7 | NC_006146.1 | + | 166525 | 0.66 | 0.939752 |
Target: 5'- gGCGUCggCCUGGGGAgcccgCggGGcCGUGg -3' miRNA: 3'- -UGCGGuaGGACUCCUa----GuaCCuGCGC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 57327 | 0.66 | 0.939752 |
Target: 5'- -gGCCucUCCcGAGGGUCAgcaUGGAgCGCc -3' miRNA: 3'- ugCGGu-AGGaCUCCUAGU---ACCU-GCGc -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 68712 | 0.66 | 0.938338 |
Target: 5'- gACGCCGUCCgcguccccgagagcUGucgcGGGGUgGUGGAgGCc -3' miRNA: 3'- -UGCGGUAGG--------------AC----UCCUAgUACCUgCGc -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 162265 | 0.66 | 0.934955 |
Target: 5'- -aGCCuguuuUCCUGGGGcUCGcugGGGCGUa -3' miRNA: 3'- ugCGGu----AGGACUCCuAGUa--CCUGCGc -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 9667 | 0.66 | 0.929922 |
Target: 5'- cUGCCGUCgUGGGaGAUguUGGcCGUGg -3' miRNA: 3'- uGCGGUAGgACUC-CUAguACCuGCGC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 121322 | 0.66 | 0.924652 |
Target: 5'- cGCGUCAUCCUgGAGGA-CAUcGAgGCc -3' miRNA: 3'- -UGCGGUAGGA-CUCCUaGUAcCUgCGc -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 114991 | 0.66 | 0.919147 |
Target: 5'- aACGCC-UCCUgGAGGAagcugCAUGGcUGCu -3' miRNA: 3'- -UGCGGuAGGA-CUCCUa----GUACCuGCGc -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 33428 | 0.66 | 0.913406 |
Target: 5'- uGCGCCgccgcucgGUCCUGGGGcUCcgGG-UGCa -3' miRNA: 3'- -UGCGG--------UAGGACUCCuAGuaCCuGCGc -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 156777 | 0.66 | 0.911638 |
Target: 5'- -aGCCAggCCcGAGGGUCAccuaaaguccccccUGGACGgGc -3' miRNA: 3'- ugCGGUa-GGaCUCCUAGU--------------ACCUGCgC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 153699 | 0.66 | 0.911638 |
Target: 5'- -aGCCAggCCcGAGGGUCAccuaaaguccccccUGGACGgGc -3' miRNA: 3'- ugCGGUa-GGaCUCCUAGU--------------ACCUGCgC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 150622 | 0.66 | 0.911638 |
Target: 5'- -aGCCAggCCcGAGGGUCAccuaaaguccccccUGGACGgGc -3' miRNA: 3'- ugCGGUa-GGaCUCCUAGU--------------ACCUGCgC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 147544 | 0.66 | 0.911638 |
Target: 5'- -aGCCAggCCcGAGGGUCAccuaaaguccccccUGGACGgGc -3' miRNA: 3'- ugCGGUa-GGaCUCCUAGU--------------ACCUGCgC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 144466 | 0.66 | 0.911638 |
Target: 5'- -aGCCAggCCcGAGGGUCAccuaaaguccccccUGGACGgGc -3' miRNA: 3'- ugCGGUa-GGaCUCCUAGU--------------ACCUGCgC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 141388 | 0.66 | 0.911638 |
Target: 5'- -aGCCAggCCcGAGGGUCAccuaaaguccccccUGGACGgGc -3' miRNA: 3'- ugCGGUa-GGaCUCCUAGU--------------ACCUGCgC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 81037 | 0.67 | 0.901223 |
Target: 5'- uCGCCccccaCUGAGGcaAUCGaGGACGCGc -3' miRNA: 3'- uGCGGuag--GACUCC--UAGUaCCUGCGC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 28276 | 0.68 | 0.874129 |
Target: 5'- -gGCCGggucucgggUCUGGGGGUCuGUGGugGUGa -3' miRNA: 3'- ugCGGUa--------GGACUCCUAG-UACCugCGC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 25198 | 0.68 | 0.874129 |
Target: 5'- -gGCCGggucucgggUCUGGGGGUCuGUGGugGUGa -3' miRNA: 3'- ugCGGUa--------GGACUCCUAG-UACCugCGC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 22120 | 0.68 | 0.874129 |
Target: 5'- -gGCCGggucucgggUCUGGGGGUCuGUGGugGUGa -3' miRNA: 3'- ugCGGUa--------GGACUCCUAG-UACCugCGC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 19042 | 0.68 | 0.874129 |
Target: 5'- -gGCCGggucucgggUCUGGGGGUCuGUGGugGUGa -3' miRNA: 3'- ugCGGUa--------GGACUCCUAG-UACCugCGC- -5' |
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28793 | 3' | -55.7 | NC_006146.1 | + | 12887 | 0.68 | 0.874129 |
Target: 5'- -gGCCGggucucgggUCUGGGGGUCuGUGGugGUGa -3' miRNA: 3'- ugCGGUa--------GGACUCCUAG-UACCugCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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