Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28798 | 3' | -48.6 | NC_006146.1 | + | 118899 | 0.66 | 0.999472 |
Target: 5'- uGCCUCAGguucGAGAGCAGguuggCgUCcACCgUCa -3' miRNA: 3'- -CGGAGUUa---CUUUCGUCa----G-AGaUGG-AG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 20021 | 0.66 | 0.999435 |
Target: 5'- gGCC-CGGUGuGccagcgucccccgcAGCcgcccAGUCUCUGCCUCc -3' miRNA: 3'- -CGGaGUUACuU--------------UCG-----UCAGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 26177 | 0.66 | 0.999435 |
Target: 5'- gGCC-CGGUGuGccagcgucccccgcAGCcgcccAGUCUCUGCCUCc -3' miRNA: 3'- -CGGaGUUACuU--------------UCG-----UCAGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 23099 | 0.66 | 0.999435 |
Target: 5'- gGCC-CGGUGuGccagcgucccccgcAGCcgcccAGUCUCUGCCUCc -3' miRNA: 3'- -CGGaGUUACuU--------------UCG-----UCAGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 13866 | 0.66 | 0.999435 |
Target: 5'- gGCC-CGGUGuGccagcgucccccgcAGCcgcccAGUCUCUGCCUCc -3' miRNA: 3'- -CGGaGUUACuU--------------UCG-----UCAGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 29255 | 0.66 | 0.999435 |
Target: 5'- gGCC-CGGUGuGccagcgucccccgcAGCcgcccAGUCUCUGCCUCc -3' miRNA: 3'- -CGGaGUUACuU--------------UCG-----UCAGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 42796 | 0.66 | 0.999284 |
Target: 5'- cGCCUCGGccagGuuGGCGGUCUCgcagagcguguagACCa- -3' miRNA: 3'- -CGGAGUUa---CuuUCGUCAGAGa------------UGGag -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 159256 | 0.66 | 0.999187 |
Target: 5'- cCCgCAAUGAGgauccuggGGUcguuGGUCUUUGCCUCu -3' miRNA: 3'- cGGaGUUACUU--------UCG----UCAGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 96714 | 0.66 | 0.999187 |
Target: 5'- uGCCUCAcgGAccagaucaacGAGCAGUUUg-ACCa- -3' miRNA: 3'- -CGGAGUuaCU----------UUCGUCAGAgaUGGag -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 16944 | 0.66 | 0.998939 |
Target: 5'- gGCC-CGGUGugccagcguccccccAGCcgcccAGUCUCUGCCUCc -3' miRNA: 3'- -CGGaGUUACuu-------------UCG-----UCAGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 21411 | 0.66 | 0.998756 |
Target: 5'- aCCUCAGaGAGGGCaggguccccauggGGgagCUCUGCUUCu -3' miRNA: 3'- cGGAGUUaCUUUCG-------------UCa--GAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 24796 | 0.67 | 0.998629 |
Target: 5'- aGCUUC-GUGAGGGgGGuguggaaauacgagaUCUUUGCCUCg -3' miRNA: 3'- -CGGAGuUACUUUCgUC---------------AGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 46288 | 0.67 | 0.998491 |
Target: 5'- aGCCcCGGUGgcGGCGGUCgagcucgagccccUCUuCCUCu -3' miRNA: 3'- -CGGaGUUACuuUCGUCAG-------------AGAuGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 126511 | 0.67 | 0.998003 |
Target: 5'- uGUCUCuccaaaAGUGGGAGCAgguagccuccuuuuuGUCUCUGCCc- -3' miRNA: 3'- -CGGAG------UUACUUUCGU---------------CAGAGAUGGag -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 4535 | 0.67 | 0.997853 |
Target: 5'- aUCUCGGUGccGGCAGcCgccgaCUACCUCc -3' miRNA: 3'- cGGAGUUACuuUCGUCaGa----GAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 80732 | 0.67 | 0.997853 |
Target: 5'- aCCUCcccUGcc-GCGGcCUCUACCUCg -3' miRNA: 3'- cGGAGuu-ACuuuCGUCaGAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 115695 | 0.67 | 0.996952 |
Target: 5'- gGUgUCGAUGGAGGCcaUCUCgugaugcACCUCa -3' miRNA: 3'- -CGgAGUUACUUUCGucAGAGa------UGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 92753 | 0.68 | 0.996023 |
Target: 5'- -aCUaCAGUGAGAGCgacgaggaaggugaaGGggcCUCUGCCUCu -3' miRNA: 3'- cgGA-GUUACUUUCG---------------UCa--GAGAUGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 45930 | 0.68 | 0.994208 |
Target: 5'- uGCCguaGGUGGAcGgGGUCUCUcuggggACCUCg -3' miRNA: 3'- -CGGag-UUACUUuCgUCAGAGA------UGGAG- -5' |
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28798 | 3' | -48.6 | NC_006146.1 | + | 65748 | 0.69 | 0.986001 |
Target: 5'- gGCCUCuuaccugcGGCGGUCUCguuagaggagGCCUCg -3' miRNA: 3'- -CGGAGuuacuu--UCGUCAGAGa---------UGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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