Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2880 | 3' | -59.2 | NC_001493.1 | + | 93450 | 0.66 | 0.743868 |
Target: 5'- cGCGgUCCAuuCGUauauuaUCCGUGGGGGGUu -3' miRNA: 3'- -CGCgAGGU--GCAgg----AGGCGCCUCCUAu -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 29310 | 0.67 | 0.714766 |
Target: 5'- aUGCUCUACGggCCgaaCGCGGGGGc-- -3' miRNA: 3'- cGCGAGGUGCa-GGag-GCGCCUCCuau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 125502 | 0.67 | 0.714766 |
Target: 5'- -aGCUCgACGUCC-CCGCGGuucGAg- -3' miRNA: 3'- cgCGAGgUGCAGGaGGCGCCuc-CUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 9948 | 0.67 | 0.714766 |
Target: 5'- -aGCUCgACGUCC-CCGCGGuucGAg- -3' miRNA: 3'- cgCGAGgUGCAGGaGGCGCCuc-CUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 91761 | 0.67 | 0.675044 |
Target: 5'- uGCGC-CCGCGaCCg--GCGGAGGAc- -3' miRNA: 3'- -CGCGaGGUGCaGGaggCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 123823 | 0.68 | 0.644863 |
Target: 5'- gGCuCUCCGgGUCCUCUcucuCGGGGGAg- -3' miRNA: 3'- -CGcGAGGUgCAGGAGGc---GCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 8269 | 0.68 | 0.644863 |
Target: 5'- gGCuCUCCGgGUCCUCUcucuCGGGGGAg- -3' miRNA: 3'- -CGcGAGGUgCAGGAGGc---GCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 87673 | 0.68 | 0.624681 |
Target: 5'- -gGCUCCuCGUacgCCGCGGGGGGc- -3' miRNA: 3'- cgCGAGGuGCAggaGGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 87727 | 0.68 | 0.624681 |
Target: 5'- -gGCUCCuCGUacgCCGCGGGGGGc- -3' miRNA: 3'- cgCGAGGuGCAggaGGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 87906 | 0.68 | 0.624681 |
Target: 5'- -gGCUCCuCGUacgCCGCGGGGGGc- -3' miRNA: 3'- cgCGAGGuGCAggaGGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 87864 | 0.68 | 0.60452 |
Target: 5'- -aGCUCCuCGUacgCCGCGGGGGGc- -3' miRNA: 3'- cgCGAGGuGCAggaGGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 126346 | 0.69 | 0.544726 |
Target: 5'- gGCGCgucCCGagaGUCUcgaCCGCGGAGGAc- -3' miRNA: 3'- -CGCGa--GGUg--CAGGa--GGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 10792 | 0.69 | 0.544726 |
Target: 5'- gGCGCgucCCGagaGUCUcgaCCGCGGAGGAc- -3' miRNA: 3'- -CGCGa--GGUg--CAGGa--GGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 78417 | 0.71 | 0.441151 |
Target: 5'- -aGuCUCCAgGUUCUcaCCGUGGAGGAUGu -3' miRNA: 3'- cgC-GAGGUgCAGGA--GGCGCCUCCUAU- -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 126167 | 0.71 | 0.432281 |
Target: 5'- cGCGgcucgaUCCGgGUCCUCCGCGGucGAg- -3' miRNA: 3'- -CGCg-----AGGUgCAGGAGGCGCCucCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 10613 | 0.71 | 0.432281 |
Target: 5'- cGCGgcucgaUCCGgGUCCUCCGCGGucGAg- -3' miRNA: 3'- -CGCg-----AGGUgCAGGAGGCGCCucCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 9296 | 0.73 | 0.342065 |
Target: 5'- uGCGuCUCCuccacgaaagauACGaggcguggcuaUCCUCCGCGGAGGAc- -3' miRNA: 3'- -CGC-GAGG------------UGC-----------AGGAGGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 124850 | 0.73 | 0.342065 |
Target: 5'- uGCGuCUCCuccacgaaagauACGaggcguggcuaUCCUCCGCGGAGGAc- -3' miRNA: 3'- -CGC-GAGG------------UGC-----------AGGAGGCGCCUCCUau -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 60299 | 0.75 | 0.285482 |
Target: 5'- cGCGC-CCGCGguaUUCCGCGGAGGuGUGa -3' miRNA: 3'- -CGCGaGGUGCag-GAGGCGCCUCC-UAU- -5' |
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2880 | 3' | -59.2 | NC_001493.1 | + | 9488 | 0.97 | 0.009452 |
Target: 5'- cGCGCUCCACGU-CUCCGCGGAGGAUAg -3' miRNA: 3'- -CGCGAGGUGCAgGAGGCGCCUCCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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