Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2880 | 5' | -50.8 | NC_001493.1 | + | 117790 | 0.65 | 0.995078 |
Target: 5'- aCGUGUUUUUCGUGuuGAGGAaaucgucuccgaucGACuuGGGa -3' miRNA: 3'- -GCAUAGAAAGCAC--CUCCU--------------CUGcgUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 2236 | 0.65 | 0.995078 |
Target: 5'- aCGUGUUUUUCGUGuuGAGGAaaucgucuccgaucGACuuGGGa -3' miRNA: 3'- -GCAUAGAAAGCAC--CUCCU--------------CUGcgUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 94534 | 0.66 | 0.99453 |
Target: 5'- -uUAUUggUCGUGGAGGucAC-CAGGa -3' miRNA: 3'- gcAUAGaaAGCACCUCCucUGcGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 42163 | 0.66 | 0.994529 |
Target: 5'- gGUGUCUcUCGgGGAucGGAcGGCGCGuGGc -3' miRNA: 3'- gCAUAGAaAGCaCCU--CCU-CUGCGU-CC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 9233 | 0.66 | 0.991002 |
Target: 5'- aGUGUCUgcgcaggaugcgUCGUcgggacaggaccggGGAGGcGGGCGUGGGg -3' miRNA: 3'- gCAUAGAa-----------AGCA--------------CCUCC-UCUGCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 124787 | 0.66 | 0.991002 |
Target: 5'- aGUGUCUgcgcaggaugcgUCGUcgggacaggaccggGGAGGcGGGCGUGGGg -3' miRNA: 3'- gCAUAGAa-----------AGCA--------------CCUCC-UCUGCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 105887 | 0.67 | 0.989028 |
Target: 5'- --aGUCUUUgaUGGAGGGGAUcCAGGc -3' miRNA: 3'- gcaUAGAAAgcACCUCCUCUGcGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 65979 | 0.67 | 0.988149 |
Target: 5'- uCGUAUcCUUUUGagGGGGGuggggcguucgucggGGACGUGGGg -3' miRNA: 3'- -GCAUA-GAAAGCa-CCUCC---------------UCUGCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 2682 | 0.67 | 0.987534 |
Target: 5'- uCGUccGUC-UUCGU-GAGGGGugGgAGGg -3' miRNA: 3'- -GCA--UAGaAAGCAcCUCCUCugCgUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 118236 | 0.67 | 0.987534 |
Target: 5'- uCGUccGUC-UUCGU-GAGGGGugGgAGGg -3' miRNA: 3'- -GCA--UAGaAAGCAcCUCCUCugCgUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 125157 | 0.67 | 0.987377 |
Target: 5'- cCGUGgguuauaacugacUUUUUCGUGuuaGGGGGGCGCGGa -3' miRNA: 3'- -GCAU-------------AGAAAGCACc--UCCUCUGCGUCc -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 9602 | 0.67 | 0.987377 |
Target: 5'- cCGUGgguuauaacugacUUUUUCGUGuuaGGGGGGCGCGGa -3' miRNA: 3'- -GCAU-------------AGAAAGCACc--UCCUCUGCGUCc -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 19608 | 0.67 | 0.984081 |
Target: 5'- aGUGUagugCG-GGGGGuGACGUGGGg -3' miRNA: 3'- gCAUAgaaaGCaCCUCCuCUGCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 20458 | 0.68 | 0.977603 |
Target: 5'- gGUGUCUccucgUCGUGuucGGGAGGguUGCAGGc -3' miRNA: 3'- gCAUAGAa----AGCACc--UCCUCU--GCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 125257 | 0.68 | 0.974261 |
Target: 5'- gGUAUCUacgugcggacccgcgCGUccaucGAGGAGAUGCGGGg -3' miRNA: 3'- gCAUAGAaa-------------GCAc----CUCCUCUGCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 9703 | 0.68 | 0.974261 |
Target: 5'- gGUAUCUacgugcggacccgcgCGUccaucGAGGAGAUGCGGGg -3' miRNA: 3'- gCAUAGAaa-------------GCAc----CUCCUCUGCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 125008 | 1.11 | 0.007071 |
Target: 5'- uCGUAUCUUUCGUGGAGGAGACGCAGGu -3' miRNA: 3'- -GCAUAGAAAGCACCUCCUCUGCGUCC- -5' |
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2880 | 5' | -50.8 | NC_001493.1 | + | 9454 | 1.11 | 0.007071 |
Target: 5'- uCGUAUCUUUCGUGGAGGAGACGCAGGu -3' miRNA: 3'- -GCAUAGAAAGCACCUCCUCUGCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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