Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28800 | 3' | -51.6 | NC_006146.1 | + | 106016 | 0.66 | 0.986644 |
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Target: 5'- -cUUUCUUCAGUccaUGcUGGC-CGUGGCc -3' miRNA: 3'- uuGAAGAAGUCGa--AC-ACCGcGUACCG- -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 125599 | 0.66 | 0.982956 |
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Target: 5'- gAGCUguUCUcCGGCUUuccaccgaggGUGGgGCAUGGg -3' miRNA: 3'- -UUGA--AGAaGUCGAA----------CACCgCGUACCg -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 55307 | 0.66 | 0.982956 |
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Target: 5'- gGACUUCUUCAGCgucuucccGGC-CGaGGCa -3' miRNA: 3'- -UUGAAGAAGUCGaaca----CCGcGUaCCG- -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 4352 | 0.68 | 0.963921 |
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Target: 5'- cAGC-UCUUCAGCgacGUGGC-CAcGGCc -3' miRNA: 3'- -UUGaAGAAGUCGaa-CACCGcGUaCCG- -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 69368 | 0.68 | 0.960317 |
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Target: 5'- gGACguagUCAGUgcccUGGCGCAUGGUu -3' miRNA: 3'- -UUGaagaAGUCGaac-ACCGCGUACCG- -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 112771 | 0.69 | 0.938591 |
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Target: 5'- uGACUUgCUUUGGC-UGUGuGCGCuUGGUg -3' miRNA: 3'- -UUGAA-GAAGUCGaACAC-CGCGuACCG- -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 66553 | 0.69 | 0.928107 |
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Target: 5'- cGAUgUCUUUgauCUUGUGGCGCA-GGCg -3' miRNA: 3'- -UUGaAGAAGuc-GAACACCGCGUaCCG- -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 133638 | 0.73 | 0.789825 |
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Target: 5'- gGACUuggCUg-GGCUuugaUGUGGUGCAUGGCc -3' miRNA: 3'- -UUGAa--GAagUCGA----ACACCGCGUACCG- -5' |
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| 28800 | 3' | -51.6 | NC_006146.1 | + | 107460 | 1.09 | 0.00657 |
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Target: 5'- aAACUUCUUCAGCUUGUGGCGCAUGGCc -3' miRNA: 3'- -UUGAAGAAGUCGAACACCGCGUACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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