Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28801 | 5' | -51.2 | NC_006146.1 | + | 41685 | 0.72 | 0.872704 |
Target: 5'- cCUGCuggcucuuUCAGCGCUCACcgGCGGGgGGu -3' miRNA: 3'- -GAUGu-------AGUUGCGAGUGa-CGUCUgCCu -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 46717 | 0.68 | 0.971306 |
Target: 5'- -gGCcgCGGCgGCUC-CgagGCGGGCGGAg -3' miRNA: 3'- gaUGuaGUUG-CGAGuGa--CGUCUGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 56492 | 0.66 | 0.992292 |
Target: 5'- -gGCAUCAGCGC--GC-GCAGuCGGGc -3' miRNA: 3'- gaUGUAGUUGCGagUGaCGUCuGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 57325 | 0.68 | 0.974118 |
Target: 5'- -gACG-CGugGCUCuCUGgGGGCGGGg -3' miRNA: 3'- gaUGUaGUugCGAGuGACgUCUGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 59396 | 0.66 | 0.995017 |
Target: 5'- gUACAU--GgGC-CACUGCAGGCuGGAu -3' miRNA: 3'- gAUGUAguUgCGaGUGACGUCUG-CCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 60616 | 0.69 | 0.961566 |
Target: 5'- cCUACGcCGAgGCcaUCAUgGCAGACGGGg -3' miRNA: 3'- -GAUGUaGUUgCG--AGUGaCGUCUGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 70663 | 0.67 | 0.985251 |
Target: 5'- gUGCGUgAACGCcccCACUGCc-ACGGAg -3' miRNA: 3'- gAUGUAgUUGCGa--GUGACGucUGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 89748 | 0.66 | 0.993308 |
Target: 5'- gUGCAUCGG-GCUC-CgGCAGugGCGGAa -3' miRNA: 3'- gAUGUAGUUgCGAGuGaCGUC--UGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 106507 | 1.1 | 0.007726 |
Target: 5'- cCUACAUCAACGCUCACUGCAGACGGAg -3' miRNA: 3'- -GAUGUAGUUGCGAGUGACGUCUGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 106634 | 0.69 | 0.965037 |
Target: 5'- uUGCcgCAGCGCcUGCUGCGGcCGGc -3' miRNA: 3'- gAUGuaGUUGCGaGUGACGUCuGCCu -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 110872 | 0.7 | 0.940643 |
Target: 5'- gCUGCGUCucGgGCUCGggcGCAGGCGGGg -3' miRNA: 3'- -GAUGUAGu-UgCGAGUga-CGUCUGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 130660 | 0.66 | 0.992292 |
Target: 5'- -aGguUCAGC-CUCGCguagGCGGACGGu -3' miRNA: 3'- gaUguAGUUGcGAGUGa---CGUCUGCCu -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 132787 | 0.78 | 0.582403 |
Target: 5'- gCUGCAggcucgCAGCuGCUCagcaGCUGCAGGCGGGg -3' miRNA: 3'- -GAUGUa-----GUUG-CGAG----UGACGUCUGCCU- -5' |
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28801 | 5' | -51.2 | NC_006146.1 | + | 151379 | 0.69 | 0.961566 |
Target: 5'- -gGCGUaCGAguccCGCU-GCUGCAGGCGGGg -3' miRNA: 3'- gaUGUA-GUU----GCGAgUGACGUCUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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