Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28805 | 5' | -46.2 | NC_006146.1 | + | 98631 | 0.66 | 0.999995 |
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Target: 5'- cGGUGacugccUUCUGGgCCUGggcaaaugACACGUc -3' miRNA: 3'- -CCACa-----AAGACCaGGACauaa----UGUGCG- -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 53009 | 0.66 | 0.999989 |
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Target: 5'- ----cUUCUGGcCCUGggccuCACGCg -3' miRNA: 3'- ccacaAAGACCaGGACauaauGUGCG- -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 63752 | 0.66 | 0.999985 |
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Target: 5'- cGUGUUUCUGGUgggcgacguagCCUauc-UGCACGUu -3' miRNA: 3'- cCACAAAGACCA-----------GGAcauaAUGUGCG- -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 9617 | 0.67 | 0.999961 |
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Target: 5'- cGUGcaUUUCUGGgggUGUAagGCACGCg -3' miRNA: 3'- cCAC--AAAGACCaggACAUaaUGUGCG- -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 67721 | 0.69 | 0.999362 |
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Target: 5'- uGGUGUgucUCUGGaCCUGUGcgucaACGUg -3' miRNA: 3'- -CCACAa--AGACCaGGACAUaaug-UGCG- -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 161599 | 0.7 | 0.997908 |
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Target: 5'- -aUGUUUCUGGUUaUGUGUUAUaauaaAUGCa -3' miRNA: 3'- ccACAAAGACCAGgACAUAAUG-----UGCG- -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 154164 | 0.74 | 0.976399 |
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Target: 5'- cGGUGgUUCUGGUUCUGUAggucCACa- -3' miRNA: 3'- -CCACaAAGACCAGGACAUaau-GUGcg -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 66027 | 0.75 | 0.959136 |
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Target: 5'- -uUGgagCUGGUCCUGUGguuaugccucagGCACGCa -3' miRNA: 3'- ccACaaaGACCAGGACAUaa----------UGUGCG- -5' |
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| 28805 | 5' | -46.2 | NC_006146.1 | + | 104908 | 1.15 | 0.014109 |
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Target: 5'- gGGUGUUUCUGGUCCUGUAUUACACGCc -3' miRNA: 3'- -CCACAAAGACCAGGACAUAAUGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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