Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 28806 | 3' | -56.8 | NC_006146.1 | + | 106274 | 0.66 | 0.867792 |
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Target: 5'- gGCGgaagacauccuccUgGCCUGCGCCaugaaccucaGCAAGGUCAu- -3' miRNA: 3'- aCGC-------------GgCGGACGCGG----------UGUUCUAGUuu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 57939 | 0.66 | 0.853131 |
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Target: 5'- gGCGCCGCCaugGUGCUGCA---UCAc- -3' miRNA: 3'- aCGCGGCGGa--CGCGGUGUucuAGUuu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 102906 | 0.66 | 0.845116 |
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Target: 5'- gGgGCCGUguggcguggCUGCGCCcCAAGAUCn-- -3' miRNA: 3'- aCgCGGCG---------GACGCGGuGUUCUAGuuu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 55554 | 0.66 | 0.836904 |
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Target: 5'- cGCGCUGCCUGUGUaguuuccuGCGAGAa---- -3' miRNA: 3'- aCGCGGCGGACGCGg-------UGUUCUaguuu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 111157 | 0.66 | 0.875198 |
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Target: 5'- aGCGCCGCUucagGCGCUcggggagGCGAGAg---- -3' miRNA: 3'- aCGCGGCGGa---CGCGG-------UGUUCUaguuu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 159548 | 0.66 | 0.875926 |
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Target: 5'- cGCGCagCGCCUGCGCCaggcccguguACAGcGUCu-- -3' miRNA: 3'- aCGCG--GCGGACGCGG----------UGUUcUAGuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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