Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28806 | 3' | -56.8 | NC_006146.1 | + | 38256 | 0.66 | 0.868542 |
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Target: 5'- gGCGCCGUgUGUGCCugGAacAUCu-- -3' miRNA: 3'- aCGCGGCGgACGCGGugUUc-UAGuuu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 24087 | 0.69 | 0.675302 |
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Target: 5'- gGCGCgGCCU-CGCCugGgacgcGGGUCGAGg -3' miRNA: 3'- aCGCGgCGGAcGCGGugU-----UCUAGUUU- -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 23735 | 0.79 | 0.218684 |
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Target: 5'- gGCGCCgGUCUGCGCCGCcgccGGGUCAAc -3' miRNA: 3'- aCGCGG-CGGACGCGGUGu---UCUAGUUu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 17555 | 0.68 | 0.755443 |
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Target: 5'- -uUGCCuaugacucCCUGCGCCGCcAGAUCAAu -3' miRNA: 3'- acGCGGc-------GGACGCGGUGuUCUAGUUu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 11523 | 0.69 | 0.705879 |
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Target: 5'- aGCGCgGCCUGCGagCugGcGGUCAGc -3' miRNA: 3'- aCGCGgCGGACGCg-GugUuCUAGUUu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 3148 | 0.66 | 0.868542 |
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Target: 5'- cGCGCCcacccggccCCUGCGCC-Cu-GAUCGAGc -3' miRNA: 3'- aCGCGGc--------GGACGCGGuGuuCUAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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