Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28806 | 3' | -56.8 | NC_006146.1 | + | 125968 | 0.75 | 0.37554 |
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Target: 5'- cGCGCC-CCUG-GCCACGAGAgguUCAAGa -3' miRNA: 3'- aCGCGGcGGACgCGGUGUUCU---AGUUU- -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 157860 | 0.75 | 0.367342 |
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Target: 5'- aGCGCCGCCuggaaaaguUGUGCCACAGGuagaggcUCGAGa -3' miRNA: 3'- aCGCGGCGG---------ACGCGGUGUUCu------AGUUU- -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 104110 | 0.75 | 0.359268 |
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Target: 5'- aUGCGCCGCCUGgGCC-CGguguccAGGUCGc- -3' miRNA: 3'- -ACGCGGCGGACgCGGuGU------UCUAGUuu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 63435 | 0.76 | 0.328225 |
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Target: 5'- aGCGCgGCCaUGCGCCACAAGcUgAAGa -3' miRNA: 3'- aCGCGgCGG-ACGCGGUGUUCuAgUUU- -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 23735 | 0.79 | 0.218684 |
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Target: 5'- gGCGCCgGUCUGCGCCGCcgccGGGUCAAc -3' miRNA: 3'- aCGCGG-CGGACGCGGUGu---UCUAGUUu -5' |
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| 28806 | 3' | -56.8 | NC_006146.1 | + | 104341 | 1.06 | 0.003257 |
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Target: 5'- cUGCGCCGCCUGCGCCACAAGAUCAAAg -3' miRNA: 3'- -ACGCGGCGGACGCGGUGUUCUAGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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