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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28815 | 5' | -51.5 | NC_006146.1 | + | 115142 | 0.66 | 0.979619 |
Target: 5'- ----cGCUgccccugGCCCGcCGCCGGgcggUGGAg -3' miRNA: 3'- auuaaCGAa------CGGGC-GUGGCCaa--ACUU- -5' |
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28815 | 5' | -51.5 | NC_006146.1 | + | 97880 | 0.67 | 0.968653 |
Target: 5'- gAGUUGCUcggUGCUCGUACCauGGcUUUGAc -3' miRNA: 3'- aUUAACGA---ACGGGCGUGG--CC-AAACUu -5' |
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28815 | 5' | -51.5 | NC_006146.1 | + | 39991 | 0.69 | 0.935377 |
Target: 5'- ---cUGCUgcgGCCCGUagugGCCGGUgUGGg -3' miRNA: 3'- auuaACGAa--CGGGCG----UGGCCAaACUu -5' |
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28815 | 5' | -51.5 | NC_006146.1 | + | 102839 | 0.7 | 0.878072 |
Target: 5'- ----cGCUUGCUCGCGCCGcucgUGGAg -3' miRNA: 3'- auuaaCGAACGGGCGUGGCcaa-ACUU- -5' |
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28815 | 5' | -51.5 | NC_006146.1 | + | 96733 | 1.05 | 0.011047 |
Target: 5'- aUAAUUGCUUGCCCGCACCGGUUUGAAg -3' miRNA: 3'- -AUUAACGAACGGGCGUGGCCAAACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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