Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2882 | 3' | -54 | NC_001493.1 | + | 125529 | 0.67 | 0.887142 |
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Target: 5'- aUGGUgaGCA--GGGAUGCgCCCGCGc -3' miRNA: 3'- aACUAgaCGUagUCCUAUG-GGGCGUa -5' |
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| 2882 | 3' | -54 | NC_001493.1 | + | 9975 | 0.67 | 0.887142 |
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Target: 5'- aUGGUgaGCA--GGGAUGCgCCCGCGc -3' miRNA: 3'- aACUAgaCGUagUCCUAUG-GGGCGUa -5' |
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| 2882 | 3' | -54 | NC_001493.1 | + | 111930 | 0.68 | 0.830994 |
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Target: 5'- -cGAauUCUGgAUCGGGAccGCCCCGUg- -3' miRNA: 3'- aaCU--AGACgUAGUCCUa-UGGGGCGua -5' |
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| 2882 | 3' | -54 | NC_001493.1 | + | 103548 | 0.7 | 0.744993 |
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Target: 5'- uUUGAUCagGUAUUuGGAgUACCCCGCGa -3' miRNA: 3'- -AACUAGa-CGUAGuCCU-AUGGGGCGUa -5' |
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| 2882 | 3' | -54 | NC_001493.1 | + | 125452 | 0.96 | 0.021891 |
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Target: 5'- gUGuAUCUGCAUCAGGAUACCCCGCAUc -3' miRNA: 3'- aAC-UAGACGUAGUCCUAUGGGGCGUA- -5' |
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| 2882 | 3' | -54 | NC_001493.1 | + | 9898 | 0.96 | 0.021891 |
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Target: 5'- gUGuAUCUGCAUCAGGAUACCCCGCAUc -3' miRNA: 3'- aAC-UAGACGUAGUCCUAUGGGGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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