Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2883 | 3' | -59.5 | NC_001493.1 | + | 94680 | 0.66 | 0.759025 |
Target: 5'- --aCCGGGUCgu-GCCCGAccggggguacgUCCCGGu -3' miRNA: 3'- agaGGCCCAGagcUGGGCU-----------AGGGCUu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 46347 | 0.66 | 0.749623 |
Target: 5'- uUCUCCGGGUgg-GACCgucuUCCCGAAu -3' miRNA: 3'- -AGAGGCCCAgagCUGGgcu-AGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 110867 | 0.66 | 0.740121 |
Target: 5'- ----aGGGUUugcgCGAUCCGGUCCCGGc -3' miRNA: 3'- agaggCCCAGa---GCUGGGCUAGGGCUu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 110354 | 0.66 | 0.729565 |
Target: 5'- aCUCCGGGcgcgggCUCGACCuucaggucaguguCGAagcccaUCCCGGg -3' miRNA: 3'- aGAGGCCCa-----GAGCUGG-------------GCU------AGGGCUu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 10672 | 0.66 | 0.720855 |
Target: 5'- cUCcCCGGG-UUCGAcaCCCGGagagUCCCGGAu -3' miRNA: 3'- -AGaGGCCCaGAGCU--GGGCU----AGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 126226 | 0.66 | 0.720855 |
Target: 5'- cUCcCCGGG-UUCGAcaCCCGGagagUCCCGGAu -3' miRNA: 3'- -AGaGGCCCaGAGCU--GGGCU----AGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 48142 | 0.66 | 0.720855 |
Target: 5'- gUCUCuCGGGUUgaagUCGACaCCcAUCCCGu- -3' miRNA: 3'- -AGAG-GCCCAG----AGCUG-GGcUAGGGCuu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 82580 | 0.66 | 0.720855 |
Target: 5'- --gCUGGGcCUgGACCaCGAUCUCGAu -3' miRNA: 3'- agaGGCCCaGAgCUGG-GCUAGGGCUu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 59888 | 0.67 | 0.711108 |
Target: 5'- cUUCaCGGG-CUaccCGACCCcuauGAUCCCGAAa -3' miRNA: 3'- aGAG-GCCCaGA---GCUGGG----CUAGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 62411 | 0.67 | 0.711108 |
Target: 5'- --gUCGGGUugaaccCUCGGCgaaCGAUCCCGGAg -3' miRNA: 3'- agaGGCCCA------GAGCUGg--GCUAGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 62245 | 0.67 | 0.701297 |
Target: 5'- gCUCCGGGaucgUUCGccgaggguucaACCCGAcacgCCCGAGc -3' miRNA: 3'- aGAGGCCCa---GAGC-----------UGGGCUa---GGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 43453 | 0.67 | 0.701297 |
Target: 5'- aUCUCCGGGgggUCGAUcugcgcggCCGAUCUCGu- -3' miRNA: 3'- -AGAGGCCCag-AGCUG--------GGCUAGGGCuu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 103042 | 0.67 | 0.701297 |
Target: 5'- ---aUGGGUC-CGAaCCGGUCCCGAc -3' miRNA: 3'- agagGCCCAGaGCUgGGCUAGGGCUu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 107606 | 0.68 | 0.620486 |
Target: 5'- aCUCCGaaGGUUggcggcgUUGACaCCGGUCCCGGGg -3' miRNA: 3'- aGAGGC--CCAG-------AGCUG-GGCUAGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 110617 | 0.68 | 0.601462 |
Target: 5'- cUCUCCuuuaccgugacGGGUCaUCGACCCGAggugcucgCCgCGAu -3' miRNA: 3'- -AGAGG-----------CCCAG-AGCUGGGCUa-------GG-GCUu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 65048 | 0.69 | 0.581522 |
Target: 5'- -gUCCGuGGuggacuucuaUCUCGACCCGAaCCUGAu -3' miRNA: 3'- agAGGC-CC----------AGAGCUGGGCUaGGGCUu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 126858 | 0.69 | 0.542107 |
Target: 5'- cCUCgGgGGUCaUCGAgcCCUGGUCCCGGGa -3' miRNA: 3'- aGAGgC-CCAG-AGCU--GGGCUAGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 11304 | 0.69 | 0.542107 |
Target: 5'- cCUCgGgGGUCaUCGAgcCCUGGUCCCGGGa -3' miRNA: 3'- aGAGgC-CCAG-AGCU--GGGCUAGGGCUU- -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 22182 | 0.7 | 0.522727 |
Target: 5'- aCUCUGGGgcaccugCUaaccCGAUCCGAUCCCGu- -3' miRNA: 3'- aGAGGCCCa------GA----GCUGGGCUAGGGCuu -5' |
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2883 | 3' | -59.5 | NC_001493.1 | + | 32619 | 0.7 | 0.507417 |
Target: 5'- -gUCCGGGgccauauccgcgCGugCCGAUCCCGGu -3' miRNA: 3'- agAGGCCCaga---------GCugGGCUAGGGCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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