Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2883 | 5' | -61.5 | NC_001493.1 | + | 30501 | 0.66 | 0.599008 |
Target: 5'- uGUCUCGGucuguCCGGGGCgaucccccuUGCCgucaACGCc -3' miRNA: 3'- -CAGAGCCu----GGCCCCGac-------ACGGg---UGUG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 102165 | 0.67 | 0.55941 |
Target: 5'- aUCUCGGACCGGGuGuCUcGaGCaCGCGCa -3' miRNA: 3'- cAGAGCCUGGCCC-C-GA-CaCGgGUGUG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 102294 | 0.67 | 0.539876 |
Target: 5'- -cCUCGGugCucaGGGCcgccUGUGCCCGCcCg -3' miRNA: 3'- caGAGCCugGc--CCCG----ACACGGGUGuG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 129007 | 0.68 | 0.524416 |
Target: 5'- aGUCUCccagGGGCCGGuagccGaucucgcaucucuuuCUGUGCCCGCACg -3' miRNA: 3'- -CAGAG----CCUGGCCc----C---------------GACACGGGUGUG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 13453 | 0.68 | 0.524416 |
Target: 5'- aGUCUCccagGGGCCGGuagccGaucucgcaucucuuuCUGUGCCCGCACg -3' miRNA: 3'- -CAGAG----CCUGGCCc----C---------------GACACGGGUGUG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 111837 | 0.69 | 0.437626 |
Target: 5'- aUCaUCagacGACCGGGGCUgGUGCUCGCGg -3' miRNA: 3'- cAG-AGc---CUGGCCCCGA-CACGGGUGUg -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 126221 | 1.06 | 0.001168 |
Target: 5'- aGUCUCGGACCGGGGCUGUGCCCACAUg -3' miRNA: 3'- -CAGAGCCUGGCCCCGACACGGGUGUG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 10666 | 1.06 | 0.001168 |
Target: 5'- aGUCUCGGACCGGGGCUGUGCCCACAUg -3' miRNA: 3'- -CAGAGCCUGGCCCCGACACGGGUGUG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 126144 | 1.09 | 0.000716 |
Target: 5'- aGUCUCGGACCGGGGCUGUGCCCACACg -3' miRNA: 3'- -CAGAGCCUGGCCCCGACACGGGUGUG- -5' |
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2883 | 5' | -61.5 | NC_001493.1 | + | 10589 | 1.09 | 0.000716 |
Target: 5'- aGUCUCGGACCGGGGCUGUGCCCACACg -3' miRNA: 3'- -CAGAGCCUGGCCCCGACACGGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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